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Key Documents

AB16502

Sigma-Aldrich

Anti-AIF Antibody, NT

Chemicon®, from rabbit

Synonyme(s) :

Apoptosis Inducing Factor

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

western blot: suitable

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... AIFM1(9131)

Spécificité

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami & Kroemer 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al. 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and is widely expressed (Susin et al. 1999).

Immunogène

Epitope: N-terminus
Rabbit anti-AIF (N-terminus of mature AIF) polyclonal antibody was raised against a peptide corresponding to amino acids 109 to 122 of human AIF (Susin et al. 1999).

Application

Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-AIF Antibody, N-terminus is validated for use in WB for the detection of AIF.
Western blot: 0.25 to 1 μg/mL.

K562 cell lysate from HeLa cells can be used as a positive control and a 67 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Liaison

Replaces: 04-430

Forme physique

Format: Purified
Purified IgG by ion exchange chromatography. Liquid in PBS containing 0.02% sodium azide.

Stockage et stabilité

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Keyu Ren et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 51(6), e7065-e7065 (2018-04-26)
Ulcerative colitis is a chronic inflammatory disease of the colon where intestinal motility is disturbed. Interstitial cells of Cajal (ICC) are required to maintain normal intestinal motility. In the present study, we assessed the effect of tumor necrosis factor-alpha (TNF-α)
Ke Zhang et al.
Molecular medicine reports, 12(3), 4415-4421 (2015-06-18)
Sepsis is one of the leading causes of mortality in severe systemic inflammatory syndrome. The endotoxin-induced inflammatory response has been linked to the development of sepsis. Rhein is a lipophilic anthraquinone isolated from Rheum rhabarbarum (rhubarb), which has a protective
Zhonghua Cheng et al.
Bioengineered, 13(1), 1359-1376 (2022-01-12)
Gastric cancer (GC), one of the most prevalent malignancies across the world, has an increasing incidence rate. Long non-coding RNA (lncRNA) PURPL (also referred to as LINC01021) has been demonstrated to influence malignant GC behaviors and partake in other cancers.
Qinqin Lin et al.
Medical science monitor basic research, 22, 165-174 (2016-12-17)
BACKGROUND Aquaporin-2 (AQP2) plays a major role in water reabsorption in the renal collecting duct, and is involved in a variety of renal disease. Recent studies have indicate that sirtuin1 (SIRT1) exerts renoprotective properties against kidney diseases. This study aimed
Yong Wang et al.
Molecular medicine (Cambridge, Mass.), 25(1), 17-17 (2019-05-08)
Obesity is one of the leading causes of insulin resistance. Accumulating reports have highlighted that serum amyloid A-1 (SAA1) is a potential candidate that is capable of attenuating insulin resistance. Hence, we conducted the current study with aims of investigating

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