70922
BugBuster® HT Protein Extraction Reagent
Synonyme(s) :
Cell lysis reagent, Protein extraction buffer
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A propos de cet article
form
liquid
Quality Segment
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
2-8°C
General description
Application
- to lyse the Escherichia coli cell pellets following centrifugation for protein expression and immunoblotting
- for protein extraction from bacterial cell pellet
- used to lyse the E. coli cell pellets for the expression and purification of dMIC60 (mitofilin) protein
Features and Benefits
- Fast protein extraction and nucleic acid degradation
- Ideal for processing various samples of different volumes
- Increased extraction is ensured by the addition of rLysozyme solution
Legal Information
Disclaimer
Classe de stockage
10 - Combustible liquids
wgk
WGK 2
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Contenu apparenté
E. coli transformation with poly-histidine tagged constructs represents a common vehicle for protein production. Initial screening of small-scale cultures is routinely performed to identify clones producing the highest amounts of protein with the desired form and function. As the demand for greater throughput at this level has increased, so has the need for process simplification and greater reproducibility. To meet this need, we have developed a condensed workflow that can be performed in a single device.
Traditionally, protein purification from E. coli consists of four distinct phases: harvest, bacterial cell lysis, lysate clarification and protein purification. Bacterial lysis typically requires several time-consuming, hands-on steps, such as freeze/thaw cycles and sonication. These harsh lysis techniques may negatively impact protein quality and contribute to sample-to-sample variability. To maintain protein activity and integrity, detergent-based lysis buffers are routinely used to avoid mechanical protein extraction methods. Regardless of the lysis method used, centrifugation is traditionally required to pellet unwanted cell debris and permit recovery of the clarified lysate. The final step, purification, is frequently performed using affinity media specific for expressed epitope tags. Agarose-based media have typically been used, either as a slurry in microcentrifuge tubes or packed into gravity-driven or spin columns. While easier to manipulate, columns are greatly affected by lysate consistency and carryover of cell debris, which can lead to clogging of the column frits.
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| 70922-4 | 04055977272765 |
| 70922-3 | 04055977272758 |
| 70922-5 | 04055977272192 |