539514

Protein Phosphatase, Lambda, Recombinant, E. coli

• Synonyme(s) : Protein Phosphatase, Lambda
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 100
• Pureté: ≥95% (SDS-PAGE)
• Forme: liquid
• Activité spécifique: ≥400,000 units/mL
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Activité étrangère: Protease, none detected
• Conditions d'expédition: wet ice
• Température de stockage: −70°C

Description

Description générale

Recombinant λ PPase expressed in E. coli. A 221-amino acid, Mn²⁺-dependent, protein phosphatase. Hydrolyzes phosphate groups on serine, threonine, tyrosine, or histidine residues. Inhibited by orthovanadate. Note: 1KU = 1,000 units.

Research Area: Cell Signaling
The protein phosphatase encoded by bacteriophage λ (λPP) is part of a family of Ser/Thr phosphatases (Ser/Thr PPases), which includes eukaryotic protein phosphatases 1 (PP1), 2A (PP2A), and 2B (calcineurin).

Application

Protein Phosphatase, Lambda, Recombinant, E. coli is suitable for the detection of phospho-KRAS.

Actions biochimiques/physiologiques

Protein phosphatases play a crucial role in embryonic development, metabolic homeostasis, stress responses, cell cycle transitions, and numerous other vital biological processes.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per min at 30°C, pH 7.8. 20-200 Units will typically remove >95% of phosphates from serine/threonine residues in 0.1 nmol of protein in 30 min. 100-1000 Units will typically remove >95% of phosphates from tyrosine in 0.1 nmol of protein in 30 min.

Forme physique

In 100 mM NaCl, 50 mM HEPES, 2 mM DTT, 0.1 mM MnCl₂, 0.1 mM EGTA, 50% glycerol, 0.01% BRIJ^® 35 Detergent, pH 7.5.

Notes préparatoires

Further dilute in 50 mM Tris-HCl, pH 7.8, 5 mM DTT, 2 mM MnCl₂, and 100 µg/ml BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Zhuo, S., et al. 1993. J. Biol. Chem.268, 17754.
Gordon, J.A. 1991. Methods Enzymol.201, 477.
Cohen, P.T.W. and Cohen, P. 1989. Biochem. J.260, 931.

Informations légales

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable