345749

GDF-8 Propeptide

≥90% (SDS-PAGE), lyophilized powder, mouse

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.26

Propriétés

• product name: GDF-8 Propeptide, His•Tag^®, Mouse, Recombinant, GDF-8 Propeptide, His•Tag, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.
• Produit recombinant: expressed in mouse NSO cells (myeloma cells)
• Niveau de qualité: 100
• Pureté: ≥90% (SDS-PAGE)
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: ≤1.0 EU/μg protein Endotoxin
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Recombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.

Application

GDF-8 Propeptide, His•Tag, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.

Actions biochimiques/physiologiques

ED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from 0.2 µm filtered PBS, BSA.

Reconstitution

Following reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 50 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Autres remarques

Hill, J.J. et al. 2003. Molecular Endocrinology17, 1144.
Thies, R.S. et al. 2001. Growth Factors18, 251.
Chang, H. et al. 2002. Endocrine Reviews23, 787.
McPherron, A.C. et al. 1997. Nature387, 83.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2