218705

Casein Kinase I Inhibitor, D4476

InSolution, ≥95%

• Synonyme(s) : InSolution Casein Kinase I Inhibitor, D4476
• Formule empirique (notation de Hill): C₂₃H₁₈N₄O₃
• Poids moléculaire : 398.41
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: liquid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic); protect from light
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

A cell-permeable, potent, and relatively specific ATP-competitive inhibitor of CK1 (IC₅₀ = 200 nM from S. pombe; 0.3 µM for CK1δ) and ALK5 (IC₅₀ = 500 nM). A weak inhibitor of p38α MAP kinase (IC₅₀ = 12 µM) and several other kinases tested, including PKB, SGK, and GSK-3β. Suppresses the site-specific phosphorylation (Ser³²² and Ser³²⁵) and nuclear exclusion of FOXO1A in H4IIE hepatoma cells. Shown to be ~10-fold more potent than IC261 (Cat. No. 400090; IC₅₀ = 2.5 µM for CK1).

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
CK1, CK1d

Product competes with ATP.

Reversible: no

Target IC₅₀: 0.2 µM, 0.3 µM against CK1, CK1d, respectively

Conditionnement

Packaged under inert gas

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Irritant (B)

Forme physique

A 10 mM (1 mg/251 µl) solution of Casein Kinase I Inhibitor, D4476 (Cat. No. 218696) in DMSO

Reconstitution

Following initial use, aliquot and refrigerate (4°C).

Autres remarques

Rena, G., et al. 2004. EMBO Reports5, 60.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany