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Key Documents

178615

Sigma-Aldrich

Anti-Aquaporin 5 Rabbit pAb

liquid, Calbiochem®

Synonyme(s) :

Anti-AQP5

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

liquid

Ne contient pas

preservative

Espèces réactives

rat, mouse, human

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Isotype

IgG

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated for ELISA, Western blotting, immunofluorescence, and immunohistochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa AQP5 protein.
Recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts.

Immunogène

a synthetic peptide corresponding to a 17-amino acid sequence in the cytoplasmic region of rat AQP5

Application

ELISA (0.5-1.0 µg/ml)

Immunoblotting (1-10 µg/ml)

Immunofluorescence (2-20 µg/ml)

Immunohistochemistry (2-20 µg/ml)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Remarque sur l'analyse

Positive Control
Epithelial cells

Autres remarques

Does not cross-react with other AQP proteins. The use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands. Variables associated with assay conditions will dictate the optimal working dilution.
Lee, M.D., et al. 1996. J. Biol. Chem. 271, 8599.
Raina, S., et al. 1995. J. Biol. Chem. 270, 1908.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

M Cristina Kenney et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 52(10), 1341-1350 (2004-09-24)
Corneas with edema-related diseases lose transparency, which causes significant vision loss. This study analyzed seven aquaporins (AQPs) in normal corneas, pseudophakic/aphakic bullous keratopathy (PBK/ABK) corneas, Fuchs' dystrophy corneas, keratoconus corneas, post-cataract surgery (PCS) corneas, and normal organ-cultured corneas. RNA levels
Amy P Wong et al.
The Journal of clinical investigation, 119(2), 336-348 (2009-01-24)
The bone marrow compartment is enriched in stem and progenitor cells, and an unidentified subpopulation of these cells can contribute to lung epithelial repair. Here we identify this subpopulation and quantitate its relative contribution to injured airway epithelium. A subpopulation
Devendra Kumar Maurya et al.
PloS one, 10(6), e0129171-e0129171 (2015-06-13)
TMEM16A/ANO1 is a calcium-activated chloride channel expressed in several types of epithelia and involved in various physiological processes, including proliferation and development. During mouse embryonic development, the expression of TMEM16A in the olfactory epithelium is dynamic. TMEM16A is expressed at
Ninche Ninche et al.
Development (Cambridge, England), 147(19) (2020-10-01)
Salivary glands exert exocrine secretory function to provide saliva for lubrication and protection of the oral cavity. Its epithelium consists of several differentiated cell types, including acinar, ductal and myoepithelial cells, that are maintained in a lineage-restricted manner during homeostasis
Lucas T Woods et al.
PloS one, 10(5), e0123641-e0123641 (2015-05-09)
Transforming growth factor-β (TGF-β) is a multi-functional cytokine with a well-described role in the regulation of tissue fibrosis and regeneration in the liver, kidney and lung. Submandibular gland (SMG) duct ligation and subsequent deligation in rodents is a classical model

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