Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

17-283

Sigma-Aldrich

Rac1 Activation Assay Kit

Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.

Synonyme(s) :

Activation Assay Kit, Rac1 Activity Kit, Rac1 Assay Kit

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.32

Niveau de qualité

100

Espèces réactives

human, rat, mouse

Fabricant/nom de marque

Upstate®

Technique(s)

activity assay: suitable
affinity binding assay: suitable

Numéro d'accès NCBI

NM_006908.3
NM_018890.2
NM_198829.1

Numéro d'accès UniProt

P63000

Conditions d'expédition

dry ice

Informations sur le gène

human ... RAC1(5879)

Description générale

Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Ras-related C3 botulinum toxin substrate 1 (Rac1)-GTP from cell lysates and detected by a Rac1 specific monoclonal antibody. Rac1 belongs to the Ras superfamily of small guanosine triphosphatases (GTPases). It is expressed in two major forms such as an inactive GDP-bound form and an active GTP-bound form. Rac1 is involved in influencing several physiological conditions by interacting with effectors that activate several signaling cascades. The RAC1 gene is located on the human chromosome at 7p22.1.

Application

Rac1 Activation Assay Kit has been used in the detection of active Ras-related C3 botulinum toxin substrate 1 (Rac1) in brain homogenates of mice and humans by immunoblotting.

Composants

Anti-Rac1, clone 23A8 (Cat.# 05-389)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)

Qualité

Routinely evaluated by precipitating Rac-GTP from 3T3 cell lysates that had been loaded with GTPγS. The precipitated Rac-GTP was detected by immunoblot analysis using anti-Rac.

Liaison

Replaces: SGT425

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

CorrosionEnvironment
GHS05,GHS09

Mention d'avertissement

Danger

Mentions de danger

H318,H410

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Anti-arrhythmic surgery for atrioventricular junctional and atrioventricular re-entrant tachycardia--a report of six cases.
Koo, C C, et al.
Annals of the Academy of Medicine, Singapore, 19, 67-72 (1990)
Incorporation of CXCR4 into membrane lipid rafts primes homing-related responses of hematopoietic stem/progenitor cells to an SDF-1 gradient.
Marcin Wysoczynski, Ryan Reca, Janina Ratajczak, Magda Kucia, Neeta Shirvaikar et al.
Blood null
Michelle E Marchese et al.
Biochemistry, 51(41), 8235-8246 (2012-09-14)
Vascular adhesion molecules regulate the migration of leukocytes from the blood into tissue during inflammation. Binding of leukocytes to vascular cell adhesion molecule-1 (VCAM-1) activates signals in endothelial cells, including Rac1 and calcium fluxes. These VCAM-1 signals are required for
The role of mechanical stretching in the activation and localization of adhesion proteins and related intracellular molecules.
Boccafoschi, F, et al.
Journal of Cellular Biochemistry, 112, 1403-1409 (2011)
rs12977 of RAC1 is associated with the progress of gastric cancer in Iranian population
Javani A, et al.
Gene Reports, 23, 101069-101069 (2021)
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique