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Merck

07-641

Anti-MAP2K1/MEK1 Antibody

Upstate, rabbit polyclonal

Synonyme(s) :

Anti-CFC3, Anti-MAPKK1, Anti-MEK1, Anti-MEL, Anti-MKK1, Anti-PRKMK1

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A propos de cet article

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, rat, human
Application:
IP, WB
Citations:
37
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Nom du produit

Anti-MEK1 Antibody, Upstate®, from rabbit

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... MAP2K1(5604)

General description

45kDa

Immunogen

Peptide corresponding to amino acids 2-18 (PKKKPTPIQLNPAPDGS) of human MEK1.

Application

Research Category
Signaling
Research Sub Category
MAP Kinases
This Anti-MEK1 Antibody is validated for use in IP, WB for the detection of MEK1.

Biochem/physiol Actions

MEK1
Predicted to cross-react with rabbit based on sequence homology.

Physical form

70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol.
Format: Purified
Protein A purified

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
Routinely evaluated by immunoblot on RIPA lysates from A431 and 3T3/A31 cells.

Other Notes

Replaces: 04-376

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Classe de stockage

10 - Combustible liquids

wgk

WGK 1



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Bacillus anthracis lethal toxin represses MMTV promoter activity through transcription factors.
Kang, Z; Webster Marketon, JI; Johnson, A; Sternberg, EM
Journal of Molecular Biology null
Yasuyo Urasaki et al.
Nutrients, 12(2) (2020-02-06)
This study used nanofluidic protein posttranslational modification (PTM) profiling to measure the effects of six cannabidiol (CBD) oils and isolated CBD on the signaling pathways of a cultured SH-SY5Y neuronal cell line. Chemical composition analysis revealed that all CBD oils
C/EBPbeta-2 confers EGF-independent growth and disrupts the normal acinar architecture of human mammary epithelial cells.
Bundy, L; Wells, S; Sealy, L
Molecular Cancer null