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DIONEKIT

Sigma-Aldrich

DioneKit

Synonyme(s) :

Microenvironment mapping technology, Photoinduced proximity labeling kit, Protein-protein interaction elucidation technology, Small molecule-photocatalyst conjugate labeling

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.32

Température de stockage

−20°C

Description générale

The Dione MicroMap Kit provides a method for small molecule intracellular target identification via photocatalytic proximity labeling. The kit contains a catalyst that can be coupled to an acid on almost any small-molecule ligand. The subsequent ligand-Iridium conjugate can be used to perform multiple target identification experiments. The following kit provides enough Ir-catalyst to synthesize 20 μMoles of iridium–ligand conjugate and enough Diazirine-PEG3-Biotin to perform 10 proteomics experiments.
The kit provides a step-by-step procedure for conjugating your ligand of choice to a photocatalyst that localizes light-mediated crosslinking to the ligands target. The ligand–iridium conjugate can then be incubated with live cells along with a biotinylated diazirine reagent. Analogous to a satellite, the photocatalyst localizes carbene generation from the diazirine only in the direct vicinity and thus covalently modifies the target protein in a catalytic manner, amplifying the signal for downstream analysis.
To link to an amine on a small molecule (instead of an acid), the Hyas Kit is available.

Photoreactors commercially available:

Application

Proximity protein labeling, protein-protein interactions, photoaffinity labeling, proteomics

Actions biochimiques/physiologiques

Kit of components packaged in individual vials for enabling photoinduced proximity labeling. The kit contains an Ir-catalyst to couple to an acid on a small molecule ligand. Kit contains Ir-catalyst and Diazirine PEG3 Biotin to perform 10 proteomics experiments. To link to an amine on a small molecule, the Dione Kit is available.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

HYASKIT

HyasKit

Code de la classe de stockage

10 - Combustible liquids

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Articles

Introduction to Target Identification with Hyas and Dione Kits

In cases where photoaffinity labeling (PAL) probes are ineffective, Hyas and Dione Kits provide an effective method for small molecule identification by using an Iridium photocatalyst tethered to the molecule under investigation.

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