X1875

Xanthine Oxidase from bovine milk

Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein

• Synonyme(s) : XOD, Xanthine:oxygen oxidoreductase
• Numéro CAS: 9002-17-9
• Numéro de classification (Commission des enzymes): 1.17.3.2.
• Numéro CE : 232-657-6
• Numéro MDL: MFCD00082145
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Type: Grade I
• Forme: ammonium sulfate suspension
• Activité spécifique: ≥0.4 units/mg protein
• Activité étrangère: uricase ≤0.5%
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Xanthine Oxidase (XOD) is a metal flavoprotein. It has flavin adenine dinucleotide (FAD), molybdenum and iron in the ratio 2:2:8. This homodimer has a molecule weight of 290kDa. It is a member of the molybdenum-protein family. This enzyme consists of two separated substrate-binding sites.

enthält auch proteolytische Aktivität

Application

Xanthine Oxidase from bovine milk has been used:

• in the preparation of xanthine oxidase (XO) solution for 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO)-spin trapping assay
• in in vitro XO assay for screening Vietnamese medicinal plants for XO inhibitory activity
• as a standard to determine XO activity
• as a standard to test the synergistic effect of docosahexaenoic acid (DHA)

Actions biochimiques/physiologiques

Hydroxylation of hypoxanthine to xanthine and xanthine to uric acid is catalyzed by xanthinebb oxidase (XO) enzyme.

Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c.

Définition de l'unité

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Forme physique

Suspension in 2.3 M (NH₄)₂SO₄ containing 1 mM sodium salicylate

Remarque sur l'analyse

Protein determined by biuret

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P304 + P340 + P312 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)