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SRP5272

Sigma-Aldrich

SIRT5, active, His tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonyme(s) :

SIR2L5

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Produit recombinant

expressed in baculovirus infected Sf9 cells

Pureté

≥70% (SDS-PAGE)

Forme

buffered aqueous glycerol solution

Activité spécifique

17.0-26.5 RLU/min·ng

Poids mol.

~39 kDa

Numéro d'accès NCBI

NM_012241

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... SIRT5(23408)

Description générale

SIRT5 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT5 being a member of class III. SIRT5 consists of eight exons and is found in two isoforms which encode a 310 aa and a 299 aa protein, respectively. Human SIRT5 is most predominantly expressed in heart muscle cells and in lymphoblasts. Fluorescence in situ hybridization analysis localized the human SIRT5 gene to chromosome 6p23. SIRT5 can deacetylate cytochrome c, a protein of the mitochondrial intermembrane space with a central function in oxidative metabolism as well as apoptosis initiation.

Actions biochimiques/physiologiques

Sirtuins are NAD+-dependent deacetylases. Sirtuin 5 (SIRT5) catalyzes the degradation of negatively charged acylated substrates. It also possesses desuccinylase, demalonylase and deglutarylase activities. The protein is overexpressed in non-small cell lung cancer (NSCLC) and may have a role in cancer growth.

Forme physique

Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol.

Notes préparatoires

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H360D

Classification des risques

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Assignment of the NAD-dependent deacetylase sirtuin 5 gene (SIRT5) to human chromosome band 6p23 by in situ hybridization.
Mahlknecht U
Cytogenetic and genome research, 112(3-4), 208-212 (2006)
Role of the Substrate Specificity-Defining Residues of Human SIRT5 in Modulating the Structural Stability and Inhibitory Features of the Enzyme.
Yu J
PLoS ONE, 11(3), e0152467-e0152467 (2016)
SIRT5 facilitates cancer cell growth and drug resistance in non-small cell lung cancer.
Lu W
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 35(11), 10699-10705 (2014)
Chemical probing of the human sirtuin 5 active site reveals its substrate acyl specificity and peptide-based inhibitors.
Roessler C
Angewandte Chemie (International Edition in English), 53(40), 10728-10732 (2014)

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