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SRP0364

Sigma-Aldrich

mTOR/Raptor/MLST8 human

recombinant, expressed in baculovirus infected Sf9 cells, ≥50% (SDS-PAGE)

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in baculovirus infected Sf9 cells

Pureté

≥50% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

137 kDa (mTOR)
 150 kDa (Raptor)
 37 kDa (MLST8)

Conditionnement

pkg of 10 μg

Fabricant/nom de marque

Sigma-Aldrich

Technique(s)

cell based assay: suitable

Solubilité

water: soluble

Numéro d'accès NCBI

NM_004958 (mTOR)
NM_020761 (Raptor)
NM_022372 (MLST8)

Application(s)

life science and biopharma

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... MLST8(64223) , MTOR(2475) , RPTOR(57521)

Description générale

This product (recombinant mTOR/Raptor/MLST8) is a complex of human mechanistic target of rapamycin (mTOR), also known as FRAP1 (GenBank Accession No. NM_004958), amino acids 1362-end with N-terminal FLAG-tag, MW = 137 kDa; human mTOR associated protein, LST8 homolog (MLST8) (GenBank Accession No. NM_022372) amino acids 2-326 (end) with N-terminal His-tag, MW = 37 kDa and human regulatory associated protein of MTOR complex 1 (Raptor) (GenBank Accession No. NM_020761) amino acids 2-1335 (end) with N-terminal His-tag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system.

Application

Recombinant mTOR/Raptor/MLST8 has been used in in vitro caspase reactions.

Actions biochimiques/physiologiques

Mechanistic target of rapamycin (mTOR) modulates cell growth and division by integrating nutrient and mitogen signals. It also modulates the functions of antigen-presenting cells, like dendritic cells and activates T-cells. mTOR is also involved in innate and adaptive immunity. mTOR associated protein, LST8 homolog (MLST8) is a subunit of both the mTOR complex 1 and 2 (mTORC1 and 2). It is crucial for the activation of the mTOR kinase and is a regulator of mTOR pathways. Regulatory-associated protein of mTOR complex 1 (RAPTOR) has an important role in modulating cell growth and energy homeostasis as a scaffolding protein. It takes part in recruiting substrates of the mechanistic target of rapamycin (mTOR) to the rapamycin-sensitive mTOR complex 1 (mTORC1). RAPTOR also associates with proteins involved in the pathway and aids in phosphorylating them.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

mTOR controls cell cycle progression through its cell growth effectors S6K1 and 4E-BP1/eukaryotic translation initiation factor 4E.
Fingar DC
Molecular and Cellular Biology, 24(1), 200-216 (2004)
Belen Rubio-Viqueira et al.
Advances in experimental medicine and biology, 587, 309-327 (2006-12-14)
The mammalian target of rapamycin (mTOR) is involved in the control of cellular growth and proliferation. Abnormal activation of signaling pathways both proximal and distal to this kinase occurs frequently in human cancer suggesting that mTOR is an attractive target
Immunoregulatory functions of mTOR inhibition.
Thomson AW
Nature Reviews: Immunology, 9(5), 324-337 (2009)
mLST8 Promotes mTOR-Mediated Tumor Progression.
Kakumoto K
PLoS ONE, 10(4), e0119015-e0119015 (2015)
Kazuhiro Nakayama et al.
Journal of physiological anthropology, 33, 38-38 (2014-12-24)
Non-shivering thermogenesis (NST) involves a substantial amount of energy expenditure in humans and, thus, contributes to reducing the risk for obesity. Molecular evolutionary studies have reported that SNPs in/near the uncoupling protein 3 gene (UCP3) and the regulatory associated protein
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