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SRE0037

Sigma-Aldrich

β-Glucuronidase from abalone

aqueous solution, β-glucuronidase ≥100,000 units/mL

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About This Item

Numéro de classification (Commission des enzymes):
3.1.2.31 (BRENDA, IUBMB)
Numéro MDL:
MFCD00130629
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

aqueous solution

Niveau de qualité

200

β-glucuronidase activity

≥100,000 units/mL

secondary activity

≤8,000 units/mL sulfatase

Température de stockage

2-8°C

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Application

Beta-glucuronidase from abalone has been used as a standard to evaluate its performance in benzodiazepine liquid chromatography-mass spectrometry (LC-MS/MS)confirmation assays.
Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine.

Actions biochimiques/physiologiques

Beta-glucuronidase is anenzyme present in several animals, plants, and bacteria. It catalyzes thehydrolysis of Beta-glucuronide conjugates and plant glucuronides.Beta-glucuronidase is a member of the glycosyl hydrolases (GHs) family 2 and is ahomo-tetrameric glycoprotein. It facilitates the removal of glucuronic acidsfrom glucuronides and aids in the liver metabolism enzymes for the excretion ofendo- and xeno-biotics.

Définition de l'unité

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Forme physique

Supplied as an aqueous solution containing 0.85% NaCl and 0.02% sodium azide

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334

Conseils de prudence

P261 - P280 - P342 + P311

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Miau-Guo Kang et al.
Journal of analytical toxicology, 43(2), 96-103 (2018-10-03)
Benzodiazepines is a large drug group used extensively to treat sleep disorders and anxiety; these drugs are frequently associated with various crimes such as murder and drug-facilitated sexual assault. With growing use and misuse of these compounds, confirmatory assays are
Paul Awolade et al.
European journal of medicinal chemistry, 187, 111921-111921 (2019-12-14)
The emergence of disease and dearth of effective pharmacological agents on most therapeutic fronts, constitutes a major threat to global public health and man's existence. Consequently, this has created an exigency in the search for new drugs with improved clinical
D H Kim et al.
Biological & pharmaceutical bulletin, 22(2), 162-164 (1999-03-17)
To prove the relationship between the fluctuation in serum beta-glucuronidase level and hepatotoxicity, an inhibitor of beta-glucuronidase from G. lucidum was isolated and its hepatoprotective activity was investigated. The ether fraction of G. lucidum, which had potent beta-glucuronidase-inhibitory activity, protected
Francesco Candeliere et al.
Frontiers in microbiology, 13, 826994-826994 (2022-03-22)
β-glucuronidases (GUS) of intestinal bacteria remove glucuronic acid from glucoronides, reversing phase II metabolism of the liver and affecting the level of active deconjugated metabolites deriving from drugs or xenobiotics. Two hundred seventy-nine non-redundant GUS sequences are known in the

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

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