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Key Documents

SAB4301134

Sigma-Aldrich

Anti-6-His antibody produced in rabbit

affinity isolated antibody

Synonyme(s) :

6 His epitope tag, Anti His Antibody Sigma, Anti His Antibody Sigma - Anti-6×His antibody produced in rabbit

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

46 kDa

Concentration

0.5 mg/mL

Technique(s)

western blot: 1:500-1:2000 (Cell Lysate)

Isotype

IgG

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.

Spécificité

The antibody detects transfected proteins contanining 6×His tag.

Immunogène

Synthetic peptide: 18XHis conjugated to KLH

Application

Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.
The Anti-6-His antibody has been used in western blotting.

Actions biochimiques/physiologiques

The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forme physique

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

O-GlcNAcylation of fumarase maintains tumour growth under glucose deficiency
Wang T, et al.
Nature Cell Biology, 19(7), 833-833 (2017)
Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation
Jiang Y, et al.
Nature Cell Biology, 17(9), 1158-1158 (2015)
The FLAG? peptide, a versatile fusion tag for the purification of recombinant proteins.
Einhauer A and Jungbauer A
Journal of Biochemical and Biophysical Methods, 49(1-3), 455-465 (2001)
Lourena E Costa et al.
International journal of molecular sciences, 20(8) (2019-04-25)
Two Leishmania infantum mimotopes (B10 and C01) identified by phage display showed to be antigenic and immunogenic for visceral (VL) and tegumentary (TL) leishmaniasis; however, their biological targets in the parasites have not been identified. The aim of the present
Jung-Ho Shin et al.
Nature communications, 7, 12612-12612 (2016-08-27)
Bacteria respond dynamically to the changes in zinc availability. Repression by the Bacillus subtilis transcription factor Zur requires Zn(II), which binds with negative cooperativity to two regulatory sites per dimer to form, sequentially, Zur2:Zn3 and Zur2:Zn4 forms of the repressor.
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