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Principaux documents

RABHRP3

Sigma-Aldrich

HRP-Streptavidin

Synonyme(s) :

HRP streptavidin, HRP-conjugated streptavidin

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Streptavidin is a biotin-binding protein.[1] Horseradish peroxidase (HRP) is a heme-containing enzyme.[2] HRP-Streptavidin consists of the protein streptavidin, as a secondary detection reagent, conjugated to the enzyme HRP.[3] The conjugate is used in ELISA to increase the detection of various kinds of targets.[4]

Application

HRP-Streptavidin has been conjugated to secondary antibodies for western blotting.[5]

Autres remarques

This item is for RAB series EIA kits.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number

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Consulter la Bibliothèque de documents

Functional Protein Microarrays in Drug Discovery (2007)
Jayendrakumar Patel et al.
Archives of virology, 165(3), 627-641 (2020-01-23)
HCV is a potential cause of viral hepatitis, which leads to blood-borne chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. Anti-HCV antibody detection assays detect HCV infection after approximately 70 days. HCV core antigen can be detected much earlier than anti-HCV
Biotin-Streptavidin Competition Mediates Sensitive Detection of Biomolecules in Enzyme Linked Immunosorbent Assay
Lakshmipriya T, et al.
PLoS ONE, 11(3), e0151153-e0151153 (2016)
Zhen Wang et al.
Electrophoresis, 42(6), 793-799 (2020-12-24)
Western blot (protein immunoblot) is a widely used analytical technique in molecular biology. Utilizing the specific recognizing primary antibody, proteins immobilized on various matrix are investigated by subsequent visualization steps, for example, by the horse radish peroxidase conjugated secondary antibody
Chantal A Vella et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 240, 110591-110591 (2019-11-02)
The purpose of this study was to investigate changes in expression of known cellular regulators of metabolism during hyperphagia (Sept) and hibernation (Jan) in skeletal muscle and adipose tissue of brown bears and determine whether signaling molecules and transcription factors
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Questions

1–3 of 3 Questions  

  1. What is the product concentration for RABHRP3?

    1 answer

    1. The product concentration for RABHRP3 is 600 µL, which is a 100X concentrated HRP-conjugated streptavidin. It should be diluted 1:100 in an appropriate diluent before use.

      Helpful?

  2. I have a question about RABHRP3. I need to know the appropriate dilution to use for this product.

    1 answer

    1. The RABHRP3 Streptavidin-HRP Conjugate is specifically intended for titered use in RAB series ELISA only. The appropriate dilution factor for the kit it is used in will be listed in the ELISA protocol.

      The exact concentration is not known and may vary from lot to lot to ensure performance in the ELISA kit. If this product is not being used in an RAB series ELISA, it would be advisable to use a different HRP conjugate.

      We have several alternative options available, but cat# 18-152 is commonly used for ELISA and Western Blots and is provided at a 1 mg size.

      Helpful?

  3. What is the difference between HRP-Strepatavidin, HRP conjugate? My application includes use in ELISA method.

    1 answer

    1. The RABHRP3 HRP-Streptavidin product is a conjugate of the two components. It is optimized for use in ELISA kits with product numbers beginning with the letters RAB. The following link displays a list of these kits: https://www.sigmaaldrich.com/US/en/search/rab*-elisa?focus=products&page=1&perpage=30&sort=relevance&term=rab%2A%20elisa&type=product

      Other HRP-Peroxidase conjugate products have not been optimized specifically for use with these kits. In addition to HRP-Peroxidase conjugate products, other pairings are available. These include secondary antibodies, Protein A & G, and lectins. For ELISA applications, secondary antibodies are the most prevalent.

      To discuss further, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section and respond with all the required information so that a member of the Technical Service team can reach out to assist further. Thank you.

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