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MTOXRCSUP

Sigma-Aldrich

RPTEC Complete Supplement

sterile-filtered, suitable for cell culture, add to 500 ML of basal medium

Synonyme(s) :

Basal media supplement for huRPTEC

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.81

Niveau de qualité

300

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Température de stockage

−20°C

Description générale

Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 30 mL of RPTEC complete media supplement which is added to basal media and other components listed below to produce 500 mL of complete media needed for optimal growth, maintenance, and culture of Sigma′s Human Renal Proximal Tubule Epithelial Cells (RPTEC). The final RPTEC complete media was developed specifically for use with Sigma′s human RPTEC control SA7K Clone cells and various knockouts (KO).

Application

RPTEC Complete Supplement has been used in SA7K cell line culture for cell characterization.

Notes préparatoires

Prepare Complete Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-Glutamine (G7513), 30 mL of RPTEC Complete Supplement (MTOXRCSUP), 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Complete Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO2 in air atmosphere.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Development and application of human renal proximal tubule epithelial cells for assessment of compound toxicity.
Li S, et al.
Current Chemical Genomics and Translational Medicine, 11, 19-19 (2017)
Marianne K Vormann et al.
The AAPS journal, 20(5), 90-90 (2018-08-16)
Proximal tubules in the kidney play a crucial role in reabsorbing and eliminating substrates from the body into the urine, leading to high local concentrations of xenobiotics. This makes the proximal tubule a major target for drug toxicity that needs

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