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MAK066

Sigma-Aldrich

Lactate Dehydrogenase Activity Assay Kit

sufficient for 500 colorimetric tests

Synonyme(s) :

LDH Activity Assay Kit, LDH Assay, Lactic Dehydrogenase Activity Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 500 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

hematological disorder; cancer

Température de stockage

−20°C

Informations sur le gène

human ... LDHA(3939) , LDHB(3945)
mouse ... LDHA(16828) , LDHB(16832)
rat ... LDHA(24533) , LDHB(24534)

Description générale

The new Lactate Dehydrogenase Assay Kit, MAK464, is now available! Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate. Cells release LDH into the bloodstream after tissue damage or red blood cell hemolysis. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage and toxicity of tissue and cells. LDH is also elevated in certain pathological conditions such as cancer. Quantification of LDH has a broad range of applications.




Application

Lactate Dehydrogenase Activity Assay Kit has been used for measuring the activity of lactate dehydrogenase (LDH) in the cell culture medium.
Lactate Dehydrogenase Activity Assay Kit has been used to determine the concentration of lactate dehydrogenase in samples.

Principe

In this kit, LDH reduces NAD to NADH, which is specifically detected by colorimetric (450 nm) assay. The LDH Activity Assay kit quantifies LDH activity in variety of biological samples. The assay is quick, convenient, and sensitive.

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Réf. du produit
Description
Tarif

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H314

Classification des risques

Eye Dam. 1 - Skin Corr. 1B

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

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Chen Q, et al.
The Journal of Biological Chemistry, jbc-M115 (2015)
Francesca Cappellini et al.
Nanomaterials (Basel, Switzerland), 10(4) (2020-04-02)
Relevant in vitro assays that can simulate exposure to nanoparticles (NPs) via inhalation are urgently needed. Presently, the most common method employed is to expose lung cells under submerged conditions, but the cellular responses to NPs under such conditions might
Sayanta Dutta et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 118, 303-316 (2018-05-16)
Arsenic is a potent inducer of several acute and chronic nephrotoxic disorders. It promotes deleterious phenomenon like oxidative stress, inflammation, cell death and altered glucose uptake leading to distorted kidney homeostasis that end up in chronic kidney disease. This study
Adjimon G Lokossou et al.
Biology of reproduction, 102(1), 185-198 (2019-07-19)
Modulation of the activation status of immune cell populations during pregnancy depends on placental villous cytotrophoblast (VCT) cells and the syncytiotrophoblast (STB). Failure in the establishment of this immunoregulatory function leads to pregnancy complications. Our laboratory has been studying Syncytin-2
Eleonora Cavallari et al.
Frontiers in oncology, 10, 497-497 (2020-05-05)
Nuclear Magnetic Resonance allows the non-invasive detection and quantitation of metabolites to be carried out in cells and tissues. This means that that metabolic changes can be revealed without the need for sample processing and the destruction of the biological
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Articles

Aerobic Glycolysis and the Warburg Effect

Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.

Aerobic Glycolysis and the Warburg Effect

Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.

Aerobic Glycolysis and the Warburg Effect

Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.

Aerobic Glycolysis and the Warburg Effect

Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.

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