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Key Documents

LLDH-RO

Roche

L-Lactate Dehydrogenase (L-LDH)

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About This Item

Numéro de classification (Commission des enzymes):
Code UNSPSC :
12352204

Source biologique

rabbit muscle

Niveau de qualité

Forme

suspension

Activité spécifique

~550 units/mg protein (at 25 °C (1,100 U/mg at 37 °C) with pyruvate as the substrate.)

Poids mol.

140,000  Da

Conditionnement

pkg of 10 mL (10127884001 [100 mg])
pkg of 2 mL (10127230001 [10 mg])
pkg of 5 mL (10127876001 [25 mg])

Fabricant/nom de marque

Roche

Technique(s)

activity assay: suitable

Couleur

white

pH

6.0-7.0

Solubilité

water: miscible

Numéro d'accès NCBI

Numéro d'accès UniProt

Application(s)

life science and biopharma

Activité étrangère

Aldolase <0.001%
GOT <0.01%
GPT <0.01%
MDH <0.01%
PK <0.001%
myokinase <0.01%

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Informations sur le gène

Description générale

L-lactate:NAD+ oxidoreductase.
L-lactate dehydrogenase catalyzes the reversible reduction of pyruvate to L-lactate.

Spécificité

L()-Lactate dehydrogenase (LDH) is specific for L()-lactate (relative rate = 100). It does not react with D(-)-lactate.LDH will also slowly oxidize glyoxylate (oxoacetate), glycerate and 3-halogen derivatives of L-lactate (rate with each, ≤ 1); 3-phosphoglycerate does not react.LDH will reduce pyruvate (2-oxopropanate; relative rate = 100) and other 2-oxoacids (e.g., 2-oxobutyrate), with rates decreasing rapidly with increasing chain length of the acid. Heart LDH reacts with 2-oxobutyrate at a greater rate than does skeletal muscle LDH.LDH will also oxidize 2,4-diketoacids (relative rate = 10). The enzyme is realtively specific for NAD(H) (relative rate = 100); NADP(H) is utilized much less efficiently (relative rate < 1). Representative Kms for (hog) skeletal muscle LDH (pH 7.5) are lactate, 8.3 mM; pyruvate, 0.12 mM; NAD, 0.10 mM; NADH, 0.012 mM.Representative Kms for (pig) heart LDH (pH 7.5) are lactate, 3.3 mM; pyruvate, 0.15 mM; NAD, 0.07 mM;NADH, 0.011 mM. In general, the concentration of pyruvate (reduction reaction) or of L-lactate (oxidation reaction) necessary to obtain a maximal rate with LDH is lower for preparations from heart tissue than for the preparations from skeletal muscle.

Application

Reduction of α-ketoacids to α-hydroxycarboxylic acids, or reverse reaction.

Séquence

LDH is a tetramer (MW = approx. 140,000 D, rabbit muscle or pig heart LDH). In mammals, there are two types of LDH subunits, M and H, with similar molecular weight (subunit MW ≈ 36,500 D) but differing amino acid composition.
Therefore, 5 electrophoretically distinguishable LDH isoenzymes, LDH-1 through LDH-5, of differing subunit composition, are found in mammals.
The molecular weight of all LDH isoenzymes is approximately the same.
– LDH-1 (H4), LDH-2 (H3M): predominant components of heart LDH
– LDH-3 (H2M2): principal component of LDH from lymphatic tissue
– LDH-4 (HM3), LDH-5 (M4): predominate in skeletal muscle or liver LDH

Note: The M subunit of LDH used to be designated the A subunit; the H subunit was the B subunit. Under the old designation, LDH-1 isoenzyme was LDH-B4.

Définition de l'unité

One unit (U) lactate dehydrogenase will reduce 1 µmol of pyruvate to L-lactate in 1 minute at +25 °C and pH 7.0.







Note: For preparations H and I, the activity is defined at +30 °C, pH 7.8. The above assay consumes 1 mol of NADH per mol of pyruvate reduced.





Unit Conversion: For the reduction reaction, pyruvate as substrate:





1 U (+25 °C) 1.5 U (+37 °C) [hog muscle LDH in glycerol].





1 U (+25 °C) 1.8 U (+37 °C) [hog muscle LDH in amm onium sulfate].





1 U (+25 °C) 1.1 U (+30 °C) 2.0 U (+37 °C) [rabbit muscle LDH].





1 U (+25 °C) 1.4 U(+30 °C) 2.5 U (+37 °C) [pig heart LDH].





1 U (+25 °C) 2.5 U (+37 °C) [beef heart LDH].

Forme physique

Suspension in 3.2 M ammonium sulfate solution, pH approximately 7

Notes préparatoires

Activator: 2-amino-2-methyl-1-propanol, diethanolamine, fluoride and heparin (oxidation reaction). Sodium sulfite (Na2SO3) will protect the enzyme from the effects of thiol-attacking reagents. Mercaptans can reverse the inhibitory effects of thiol-attacking reagents.

Remarque sur l'analyse

Absorbance:The purified enzyme from beef heart has an absorbance of 14.9 (10 mg/ml, 280 nm).

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


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Consulter la Bibliothèque de documents

L-Lactate dehydrogenase: substrate specificity and use as a catalyst in the synthesis of homochiral 2-hydroxy acids.
Kim Mahn Joo
Journal of the American Chemical Society, 110.9, 2959-2964 (1988)
Agata M Pudlik et al.
Journal of bacteriology, 193(3), 706-714 (2010-12-01)
Carbohydrate/citrate cometabolism in Lactococcus lactis results in the formation of the flavor compound acetoin. Resting cells of strain IL1403(pFL3) rapidly consumed citrate while producing acetoin when substoichiometric concentrations of glucose or l-lactate were present. A proton motive force was generated
Enrico Desideri et al.
Autophagy, 10(9), 1652-1665 (2014-07-22)
Increased glycolytic flux is a common feature of many cancer cells, which have adapted their metabolism to maximize glucose incorporation and catabolism to generate ATP and substrates for biosynthetic reactions. Indeed, glycolysis allows a rapid production of ATP and provides

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