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HT501128

Solution de formol, tamponnée à pH neutre (10 %)

histological tissue fixative

Synonyme(s) :

Formaldéhyde, Méthanal

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A propos de cet article

UNSPSC Code:
12352114
PubChem Substance ID:
NACRES:
NA.47
MDL number:
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biological source

synthetic

Quality Segment

concentration

10% formalin (approx. 4% formaldehyde)

color

colorless

pH

6.90-7.10

density

1.080 g/cm3

application(s)

hematology
histology

storage temp.

room temp

SMILES string

[O-]#[C+H2]

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

General description

Le formol tamponné neutre (NBF) à 10 % est le fixateur le plus utilisé dans le monde pour la microscopie optique. C'est un fixateur plutôt doux. Adapté aux laboratoires à haut débit, le NBF à 10 % nécessite un temps de fixation relativement court ; il peut cependant être utilisé pour la conservation de tissus à long terme. La fixation au formol s'effectuerait entre les aldéhydes et les protéines, créant un gel qui maintiendrait les constituants cellulaires dans leurs relations in vivo. Un tissu correctement fixé doit être capable de résister à des étapes de traitement ou de coloration histologique ultérieures.

Application

Le formol en solution à 10 % tamponnée à pH neutre est un fixateur histologique universel couramment utilisé pour fixer les tissus.
Le formol en solution à 10 % tamponnée à pH neutre a été utilisé dans des colorations à l'huile rouge O pour la différenciation de fibroblastes embryonnaires de souris et de préadipocytes 3T3-L1 en adipocytes. Il a été utilisé pour l'analyse histologique d'œil de lapin et d'intestin de rat diabétique.
Il s'agit d'un fixateur histologique universel d'usage courant.

Physical form

Solution tamponnée à pH neutre.

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Cet article
HT5012F5554HT501640
description

histological tissue fixative

description

case of 24 × 60 mL, histological tissue fixative

description

histological fixative, pH 6.9-7.1

description

histological tissue fixative

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

pH

6.90-7.10

pH

6.90-7.10

pH

6.90-7.10

pH

6.90-7.10

concentration

10% formalin (approx. 4% formaldehyde)

concentration

10% formalin (approx. 4% formaldehyde)

concentration

-

concentration

10% formalin (approx. 4% formaldehyde)

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

color

colorless

color

colorless

color

colorless

color

colorless

biological source

synthetic

biological source

synthetic

biological source

-

biological source

-


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Pictogrammes

Health hazardExclamation mark

Mot-clé

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

Que se passe-t-il ?

WGK 3

EPI (équipement de protection individuelle)

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter



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Questions

1–7 of 7 Questions  
  1. Does HT501128 contain sodium phosphate monobasic or sodium phosphate dibasic? If so, what is the final % W/V? Also what is the pH of HT501128 at 25C? Thank you

    1 answer
    1. The exact buffer concentration for HT511128 is not stated on the website and is considered confidential. The classic textbook formulation for 10% Neutral Buffered Formalin is as follows:

      100 ml Formaldehyde, 37-40%
      900 ml distilled water
      4.0 grams sodium phosphate, monobasic
      6.5 grams sodium phosphate, dibasic (anhydrous)

      The Specification Sheet states the pH range must be between 6.90 to 7.10. The temperature at which the pH is not measured but should be either 20° or 25°C.

      Helpful?

  2. Is the formalin sensitive to light?

    1 answer
    1. Neutral buffered formalin solutions are particularly sensitive to ambient light. Long term exposure to UV light may result in degradation. This product is packaged in natural opaque plastic. Note that solutions should be tightly closed during storage.

      Helpful?

  3. Does Formalin solution, neutral buffered, contain any alcohol?

    1 answer
    1. Yes, this product contains Methanol which is included in the formaldehyde starting material. The concentration of methanol in this product is 1.0 - 1.5%.

      Helpful?

  4. Can the product HT501128-4L, Formalin solution, neutral buffered, 10%, be used for immunofluorescence staining?

    1 answer
    1. It is generally expected that if tissues are left in 10% Neutral Buffered Formalin for extended periods, the tissue may not be suitable for immunofluorescence. However, if the tissue spends only a short time in the 10% NBF, it might still be usable for immunofluorescence.

      Helpful?

  5. Is there a 10% neutralized buffered formalin stating the osmolality?

    1 answer
    1. None of the formalin solutions are tested for osmolality. Osmolality is significant for live cells, as cells struggle to survive when their surroundings are vastly different from their own, on the other hand formalin is only a fixative solution. Formaldehyde solutions are buffered to prevent the formation of formalin pigment or acid hematin that forms when tissues are fixed at low pH.

      Helpful?

  6. What are the differences between products 1004960700 and HT501128?

    1 answer
    1. Products 1004960700 (Formaldehyde solution, 4% buffered) and HT501128 (10% Neutral Buffered formalin) do not have a substantial difference. With HT501128, the formaldehyde used for dilution has a formaldehyde content of 37 to 40%. When the product is diluted 1 to 10 with water, it is referred to as 10% formalin, which has a formaldehyde content of 4% formaldehyde. Both products have the same concentration of formaldehyde in solution.

      Helpful?

  7. Is this product HT501128-4L is RNase/nuclease free?

    1 answer
    1. Currently, none of the formalin solutions are nuclease free. Products that are exo or endo nuclease free are typically labeled as suitable for "molecular" use. If no testing is shown as being performed, the products are not claimed to be nuclease free.

      Helpful?

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