No, if using a 95% ethanol step before eosin staining, it is important to avoid dragging water into the alcoholic eosin solution. Introducing too much water might make the solution more alkaline, which can affect the staining. pH is not typically measured as it tends to stain the pH electrode. Additionally, because this is a colored solution, using pH papers is not a viable option. If staining is poor, the solution either needs to be replaced, or it is possible to add 0.5 mL of glacial acetic acid per 100 ml of eosin Y solution to improve the staining.
HT110116
Eosin Y Solution, Alcoholic
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About This Item
Code UNSPSC :
41116124
Nomenclature NACRES :
NA.47
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Forme
solution
Niveau de qualité
Durée de conservation
Expiry date on the label.
IVD
for in vitro diagnostic use
Concentration
0.5 % (w/v) in acidified ethanol
Application(s)
hematology
histology
Température de stockage
room temp
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Application
General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
Autres remarques
Certified Eosin Y, 0.5% (w/v) in acidified 90% ethanol
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Sens. 1
Code de la classe de stockage
3 - Flammable liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
55.4 °F - closed cup
Point d'éclair (°C)
13 °C - closed cup
Équipement de protection individuelle
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Does the Eosin Y alcoholic solution (HT1101) need to be pH tested regularly?
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