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Merck
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Key Documents

H5413

Millipore

HIS-Select® Elution Buffer

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About This Item

Code UNSPSC :
12161703
Nomenclature NACRES :
NA.56

Niveau de qualité

200

Description générale

Elution buffer is a major solvent in affinity chromatography. Elution buffer is used to wash away unbound proteins at first and at a greater concentration it releases the desired protein from the ligand. It is important that the elution buffer works quickly without changing the function or activity of the desired protein.

Application

HIS-Select® Elution Buffer has been used in the elution of recombinant coagulase protein and tomoregulin 

Forme physique

Contains 250 mM Imidazole, 0.3 M sodium chloride, 50 mM sodium phosphate, pH 8.0.

Informations légales

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H360D

Classification des risques

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Hyun-Seok Hong et al.
Journal of Alzheimer's disease : JAD, 48(3), 731-743 (2015-09-25)
Amyloid-β (Aβ) protein causes neurotoxicity and its abnormal aggregation into amyloid is a pathological hallmark of Alzheimer's disease (AD). Cellular proteins able to interact with Aβ or its precursor, AβPP (amyloid-β protein precursor), may regulate Aβ production and neurotoxicity. We
Affinity Chromatography
Scouten, William H
Chromatography (1981)
Alaa H Sewid et al.
Infection and immunity, 86(8) (2018-06-13)
Coagulase activation of prothrombin by staphylococcus induces the formation of fibrin deposition that facilitates the establishment of infection by Staphylococcus species. Coagulase activity is a key characteristic of Staphylococcus pseudintermedius; however, no coagulase gene or associated protein has been studied
Pär Comstedt et al.
PloS one, 9(11), e113294-e113294 (2014-11-20)
There is currently no Lyme borreliosis vaccine available for humans, although it has been shown that the disease can be prevented by immunization with an OspA-based vaccine (LYMErix). Outer surface protein A (OspA) is one of the dominant antigens expressed

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