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F3133

Sigma-Aldrich

Fibroblast Growth Factor from bovine pituitary

lyophilized, suitable for cell culture

Synonyme(s) :

FGF

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About This Item

Numéro CAS:
62031-54-3
Numéro MDL:
MFCD00166724
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

product name

Fibroblast Growth Factor from bovine pituitary, suitable for cell culture

Source biologique

bovine pituitary glands

Niveau de qualité

200

Forme

lyophilized powder

Puissance

1-100 ng/mL

Poids mol.

16.4 kDa

Conditionnement

pkg of 10 and 5X10 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Couleur

white to off-white

Température de stockage

−20°C

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Catégories apparentées

Description générale

Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34kDa and they possess a conserved sequence of 120 amino acids.

Application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor from bovine pituitary has been used in in vitro testicular cell culture. It has also been used as a component of myogenic culture medium.

Actions biochimiques/physiologiques

Fibroblast growth factors (FGF) actively participate in cellular responses including embryogenesis and organogenesis. It specifically controls cell proliferation, differentiation and migration. FGF is known to regulate nervous system functions, tissue repair mechanism and wound healing. It also controls angiogenesis of tumor cells. Abnormal FGF mediated signaling induces tumor angiogenesis, by stimulating cancer cell proliferation and survival. FGFs carries out its function by mediating through four receptor tyrosine kinases : FGF-receptors FGFR1, FGFR2, FGFR3 and FGFR4.

Qualité

Although the major mitogenic component of this product is FGF2, the activity per unit weight of protein is less than that of purified FGF2.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.1 mg/ml BSA.

Notes préparatoires

Purified glands by a modification of the method of Gospodarowicz.

Remarque sur l'analyse

The biological activity is measured using a 3-day MTT assay.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Modulation of osteogenic, adipogenic and myogenic differentiation of mesenchymal stem cells by submicron grooved topography
Wang PY, et al.
Journal of Materials Science. Materials in Medicine, 23(12), 3015-3028 (2012)
Characterization and In Vitro Differentiation of Korean Ring-Necked Pheasant (Phasianus colchicus) Male Germ Cells
Jeong DK, et al.
Journal of Embryology transfer , 29(4), 351-359 (2014)
Cellular signaling by fibroblast growth factor receptors
Eswarakumar VP, et al.
Cytokine & Growth Factor Reviews, 16(2), 139-149 (2005)
Rocky S Tuan et al.
The Journal of the American Academy of Orthopaedic Surgeons, 21(5), 303-311 (2013-05-03)
Cartilage damaged by trauma has a limited capacity to regenerate. Current methods of managing small chondral defects include palliative treatment with arthroscopic débridement and lavage, reparative treatment with marrow-stimulation techniques (eg, microfracture), and restorative treatment, including osteochondral grafting and autologous
Jin Gyoung Jung et al.
Biology of reproduction, 76(1), 173-182 (2006-10-13)
We recently succeeded in inducing germline transmission by transferring chicken testicular cells into heterologous testes. This study was designed subsequently to identify pluripotent cells in the testicular cells, which would induce the germline transmission. Testicular cells retrieved from juvenile (4-wk-old)
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Articles

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