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Principaux documents

F1418

Sigma-Aldrich

Polysucrose 400

BioXtra, Type 400-DL, lyophilized powder

Synonyme(s) :

Polysucrose 400

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352201
Nomenclature NACRES :
NA.25

Type

Type 400-DL

Niveau de qualité

Gamme de produits

BioXtra

Forme

lyophilized powder

Impuretés

≤0.0005% Phosphorus (P)
≤0.1% Insoluble matter

Résidus de calcination

<0.1%

Couleur

white

Solubilité

water: soluble

Traces d'anions

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

Traces de cations

Al: ≤0.0005%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.001%
NH4+: ≤0.05%
Na: ≤0.01%
Pb: ≤0.001%
Zn: ≤0.0005%

Température de stockage

15-25°C

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Application

Utilisé pour la préparation des cellules et l′isolement des organelles
Ficoll® 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque, a density mixture, used for the separation of cells and organelles, especially lymphocytes and monocytes from blood. Ficoll 400® may be used for the development of new cell and organelle separation systems. Ficoll 400 is used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations.

Autres remarques

Un polymère synthétique non ionique du sucrose
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Informations légales

Ficoll is a registered trademark of Cytiva

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Soluble form of the endothelial adhesion molecule CD146 binds preferentially CD16+ monocytes.
Garibaldi S, Barisione C, Ghigliotti G, et al.
Molecular Biology Reports, 39(6), 6745-6752 (2011)
Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines.
Kishimoto S, Ishihara M, Kanatani Y, et al.
Journal of Tissue Engineering, 2, doi: 10-doi: 10 (2011)
Fahad M Almajid
Indian journal of pathology & microbiology, 54(4), 775-781 (2012-01-12)
Seronegative brucellosis is occasionally encountered in clinical practice especially in localized disease where diagnosis is reached mainly through positive blood culture. Cellular immune responses are pivotal for protection against intracellular bacteria such as Brucella. This study was performed to evaluate
Bo Chen et al.
Tissue engineering. Part C, Methods, 19(8), 586-595 (2013-02-21)
Macromolecular crowding (MMC) has been shown to have a beneficial effect on production and maturation of extracellular matrices in a monolayer cell culture model. To explore its potential in tissue engineering, a mixture of Ficoll 70 and Ficoll 400 is
Xumeng Ge et al.
PloS one, 6(12), e28707-e28707 (2011-12-17)
Cell-free protein expression (CFPE) comprised of in vitro transcription and translation is currently manipulated in relatively dilute solutions, in which the macromolecular crowding effects present in living cells are largely ignored. This may not only affect the efficiency of protein

Protocoles

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

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