HEPM

90120505, human palate epithelium. Established from palatal shelves.

• Code UNSPSC : 41106514

Propriétés

• product name: HEPM, 90120505
• Source biologique: human mouth (embryonic)
• Mode de croissance: Adherent
• Caryotype: 2n = 46, diploid
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human embryonic palatal mesenchyme

Description de la lignée cellulaire

Established from embryonic palatal shelves. The cells are very sensitive to the stimulating effect of EGF with respect to proliferation, incorporation of thymidine and ornithine decarboxylase.

Application

Proliferation studies

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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