CACO-2 Cell Line human

86010202, human colon (Caucasian colon adenocarcinoma), Epithelial

• Synonyme(s) : Caco 2 Cells, Caco-II Cells, Caco2 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: CACO-2 Cell Line human, from human colon(Caucasian colon adenocarcinoma), 86010202
• Source biologique: human colon (Caucasian colon adenocarcinoma)
• Mode de croissance: Adherent
• Caryotype: Hypertetraploid, modal no. 96
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

The CACO-2 cell line is a widely used in vitro model for mimicking the small intestine for drug transport studies. These cell lines form monolayers and are useful in intestinal permeation studies for screening excipients in drug formulations.

Origine de la lignée cellulaire

Human Caucasian colon adenocarcinoma

Description de la lignée cellulaire

Isolated from a primary colonic tumour in a 72-year-old Caucasian male using the explant culture technique. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice.

Application

CACO-2 Cell Line human has been used:

• to study if components of the sigma B regulon in Listeria monocytogenes contribute to cell invasion
• for functional analysis of bacteriocin divercin AS7
• to study the intracellular invasion by Listeria ivanovii
• to study the effect of Maslinic acid, a pentacyclic triterpene, on colon cancer cell lines
• in in vitro digestive enzymes toxicity studies
• for Hazara virus infection and cultivation
• to test the anti-cancer activity of the Curcuma longa and Origanum marjorana
• in biocompatibility studies with the bark extracts of Salix spp.
• to test the effect of N-3-oxo-dodecanoyl-homoserine (3O-C12-HSL) lactone from P.aeruginosa on the mitochondrial functionality
• to test the effect of elastin-like recombinamer (ELR) based nanoparticle
• as an in vitro epithelial cell model for studying the toxicity of zinc oxide nanoparticle
• in the intestinal permeability assays with green rooibos extract
• in toxicity studies with mycotoxin Deoxynivalenol (DON) cadmium (Cd)

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13,14
D16S539: 12,13
D5S818: 12,13
D7S820: 11,12
THO1: 6
TPOX: 9,11
vWA: 16,18

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum FBS / FCS.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsinor Trypsin/EDTA; 5% CO₂; 37°C. NB: During routine subculture the cells should always be subcultured before they achieve confluence. Cells may show the appearance of circular vacuoles in the cytoplasm. These may increase in frequency as the culture density increases to confluence. To reduce their frequency, media change confluent cultures after 2-3 days if not subcultured. Cells can clump if not separated into a single cell suspension when split.

Autres remarques

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NaviCyte Scientific holds the exclusive commercial distribution rights to the CACO-2 cell line deposited by the Memorial Sloan-Kettering Cancer Center. Note: All uses of Catalogue Numbers 86010202 and 09042001, other than for research by a non-commercial or academic entity, require a license and use authorization from NaviCyte Scientific under its exclusive arrangement with Memorial Sloan-Kettering Cancer Center. For information on the licensing terms, please contact NaviCyte Scientific via contact@navicyte-scientific.com or (+1) 973-868-6100.