CB0050
CelLytic™ B Plus Kit
For bacterial lysis
Synonyme(s) :
Cell Lysis Kit, Lysis Reagent
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About This Item
Produits recommandés
Niveau de qualité
Utilisation
kit sufficient for 5 g fresh or frozen cell paste lysis.
Technique(s)
protein extraction: suitable
Conditions d'expédition
wet ice
Température de stockage
−20°C
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Description générale
The CelLytic™ B Plus Kit is designed to efficiently lyse cells and extract proteins from both Gram negative, and difficult to lyse, Gram positive bacteria. This is accomplished using the standard CelLytic B, a proprietary non-ionic detergent in concert with lysozyme, Benzonase®, and protease inhibitors. This complete kit takes the guesswork out of protein extraction from a variety of bacterial species.
Application
This kit is compatible with FLAG®, HIS-Select®, and glutathione S-transferase based affinity chromatography protein purification systems. The detergent included in the CelLytic B Lysis Reagent can be removed from the protein by dialysis or ammonium sulfate precipitation, if necessary. The final purity of the recombinant product is usually higher than that obtained from traditional extraction methods. This is because the crude protein solution containing the non-ionic detergent eliminates much of the non-specific binding that occurs during chromatography. The mild detergent does not interfere with many enzyme assays or protein assays.
Caractéristiques et avantages
- Lyse Gram positive and Gram negative bacteria
- More efficient than sonication
- Compatible with affinity purification
- Isolate inclusion bodies for subsequent solubilization
- Non-denaturing cell lysis preserves protein function
Autres remarques
The CelLytic™ B Plus Kit contains all of the reagents and chemicals necessary to lyse both Gram-negative and Gram-positive bacteria. This kit also includes protease inhibitors to help prevent the proteolytic breakdown of proteins.
Informations légales
Benzonase® Nuclease is manufactured by Merck KGgA, Darmstadt, Germany and its affiliates. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany.
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- B7435CelLytic™ B Cell Lysis Reagent, For bacterial cell lysis, standard strengthFDS
- E1014Benzonase® Nuclease, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solutionFDS
- P8849Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solutionFDS
- L3790Lysozyme from chicken egg white, 10 mg/mLFDS
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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