A2237
Phosphatase, Alkaline shrimp
≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host
Synonyme(s) :
Phosphatase, Alkaline from shrimp, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Produit recombinant
expressed in proprietary host
Niveau de qualité
Forme
buffered aqueous glycerol solution
Concentration
≥900 DEA units/mL
Activité étrangère
RNAse, DNAse, none detected
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Description générale
Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.
Application
Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.
Actions biochimiques/physiologiques
Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.
Définition de l'unité
One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.
Forme physique
solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2
Remarque sur l'analyse
Protein determined by biuret
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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