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51411C

SAFC

Milieu essentiel minimum d′Eagle

with Earle′s Balanced Salts, with 2.0 mM L-glutamine, liquid, sterile-filtered, suitable for cell culture

Fabrication pharma

Synonyme(s) :

MEM

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Description

for research or for further manufacturing use

Niveau de qualité

500

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Composants

Earle’s salts (5% CO2): yes
phenol red: 10.62 mg/L
NaHCO3: 2200 mg/L
L-glutamine: 290 mg/L

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro . MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle′s Balanced Salts, which make them suitable for use in atmospheres charged with CO2.

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Supplément

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Description
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Code de la classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000363729
0000363729
0000333311
0000333311
SLCN8259

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Dariusz Dąbruś et al.
International journal of molecular sciences, 21(11) (2020-06-14)
This research aimed to assess the impact of cisplatin, depending on the concentration and exposure time, on the expression pattern of leptin in an endometrial cancer cell line. Ishikawa endometrial cancer cell cultures were incubated with cisplatin, at concentrations of
Tatsuhiro Tsujimoto et al.
World journal of gastroenterology, 15(44), 5533-5540 (2009-11-26)
To investigate the possible use of the multiple cytokine production modulator, Y-40138, as a novel immunotherapy in the rat nonalcoholic steatohepatitis (NASH) model. We allocated 6-wk-old male F344 rats to choline-supplemented, L-amino acid-defined (CSAA) diet (control group), CSAA diet +
Wenxin Wu et al.
Virology, 396(2), 178-188 (2009-11-17)
We studied cytokine responses to influenza virus PR8 (H1N1) and Oklahoma/309/06 (OK/06, H3N2) in a novel human lung tissue model. Exposure of the model to influenza virus rapidly activated the mitogen-activated protein kinase signaling (MAPK) pathways ERK, p38 and JNK.
Chia-Chyi Liu et al.
PloS one, 3(3), e1810-e1810 (2008-03-20)
This work investigated the replication kinetics of the four dengue virus serotypes (DEN-1 to DEN-4), including dengue virus type 4 (DEN-4) recovered from an infectious cDNA clone, in Vero cells and in MRC-5 cells grown on Cytodex 1 microcarriers. DEN-1
Robert Nason et al.
Journal of the Association for Research in Otolaryngology : JARO, 10(2), 151-160 (2009-01-16)
Osteoclasts are the only cells capable of carrying out bone resorption and therefore are responsible for the osteolysis seen in infectious diseases such as chronic otitis media and infected cholesteatoma. Pseudomonas aeruginosa is the most common organism isolated from these
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Questions

  1. Does product 51411C MEM have non essential amino acids (NEAA)?

    1 answer

    1. No, this medium does not contain non-essential amino acids (NEAA).

      Please see the link below to review the formulation:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/183/383/p51411.pdf

      Should NEAA be required, please see product M7145, a sterile 100X MEM NEAA solution for cell culture:
      https://www.sigmaaldrich.com/product/sigma/m7145

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