10107921001
Roche
Nuclease S7
Micrococcal nuclease, from Staphylococcus aureus
Synonyme(s) :
s7 nuclease
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About This Item
Code UNSPSC :
12352204
Produits recommandés
Source biologique
Staphylococcus aureus
Niveau de qualité
Forme
lyophilized
Activité spécifique
~15,000 U/mg
Conditionnement
pkg of 15,000 U
Fabricant/nom de marque
Roche
pH optimal
7.5
Conditions d'expédition
wet ice
Catégories apparentées
Application
- Removal of endogenous RNA from in vitro translation systems
- RNA sequencing experiments
Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.
Actions biochimiques/physiologiques
Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca2+ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.
Définition de l'unité
One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A260.
Stockage et stabilité
Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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