MABS2253

Anti-LRP1 α-chain Antibody, clone 8G1

• Synonyme(s) : Anti-LRP1 Antibody
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 8G1, monoclonal
• Poids mol.: calculated mol wt 504.61 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μg
• Technique(s): ELISA: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Séquence de l'épitope: N-terminal
• Numéro d'accès Protein ID: NP_002323
• Numéro d'accès UniProt: Q07954
• Conditions d'expédition: 2-8°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Prolow-density lipoprotein receptor-related protein 1 (UniProt Q07954; also known as A2MR, Alpha-2-macroglobulin receptor, APER, Apolipoprotein E receptor, CD19. LRP-1) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID: 4035) in human. LRP-1 is single pass type I membrane protein that is expressed in most tissues, but is abundant in liver, brain, and lung. It serves as a major VSMC receptor with a crucial role in maintaining vessel wall integrity and its expression in adult smooth muscle cells is shown to be essential for maintaining a contractile phenotype. LRP1 is synthesized with a signal peptide sequence (aa 1-19) and is processed in trans-Golgi network by Furin to generate a 515 kDa a subunit and an 85 kDa b subunit that contains the 100-amino intracellular domain (LRPICD; aa 4445-4544). The a and b subunits are non-covalently linked during LRP1 transport to the cell membrane. LRP1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (ApoE), APP and amyloid b. It is the primary receptor mediating transport of amyloid b peptides across the blood-brain barrier into circulation, thereby clearing them from the brain. LRP1 is required for early embryonic development and is involved in cellular lipid homeostasis and plasma clearance of chylomicron remnants and activated LRPAP1 (a2-macroglobulin). Excessive copper accumulation in the brain has been linked with reduced LRP1 mediated clearance of amyloid b peptides across the blood brain barrier, which may contribute to complications of Alzheimer′s disease.

Spécificité

Clone 8G1 is a mouse monoclonal antibody that detects human LRP-1. It targets an epitope within the a chain in the extracellular domain.

Immunogène

Full-length LRP-1 purified from human placenta and 515 kDa band excised from gel and used as immunogen.

Application

Quality Control Testing

Evaluated by Immunocytochemistry in MCF-7 cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LRP1 a-chain in MCF-7 cells.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected LRP1 -chain in Immunocytochemistry applications (Craig, J., et al. (2013). PLoS One. 8(7):e70432).

Immunoprecipitation Analysis: A representative lot immunoprecipitated LRP1 -chain in Immunoprecipitation applications (Ranganathan, S., et al. (2011). J. Biol. Chem.286(35):30535-30541; Boye, K., et al. (2017). Nat. Commun. 8(1):1571).

Western Blotting Analysis: A representative lot detected LRP1 -chain in Western Blotting applications (Strickland, D.K., et al. (1990). J Biol Chem.;265(29):17401-4; Yamamoto, K., et al. (2013). FASEB J.;27(2):511-21; Yamamoto, K. et al. (2016). Matrix Biol.;56:57-73; Boye, K., et al. (2017). Nat Commun.;8(1):1571).

ELISA Analysis: A representative lot detected LRP1 -chain in ELISA applications (Ranganathan, S., et al. (2011). J. Biol. Chem. 286(35):30535-3054).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-LRP1 a-chain, clone 8G1, Cat. No. MABS2253, is a mouse monoclonal antibody that detects LRP1 a -chain and is tested for use in ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Forme physique

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable