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Key Documents

AB10518

Sigma-Aldrich

Anti-DARPP-32 Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

protein phosphatase 1, regulatory (inhibitor) subunit 1B, dopamine and cAMP-regulated neuronal phosphoprotein 32, dopamine and cAMP regulated phosphoprotein, protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotei

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

rat, mouse, rhesus macaque

Réactivité de l'espèce (prédite par homologie)

canine (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology)

Technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

Numéro d'accès NCBI

NP_659077

Numéro d'accès UniProt

Q60829

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Ppp1R1B(19049)

Description générale

Dopamine- and cAMP-regulated neuronal phosphoprotein (DARP-32) is a cytoplasmic member of the protein phosphatase inhibitor 1 family. DARP-32 is a multifunctional protein kinase and protein phosphatase regulator, depending on the site of its activation. It possesses at least four sites that can be phosphorylated. Phosphorylation by PKA at Thr34 stimulates DARP-32 to act as a protein-phosphatase-1 (PP-1) inhibitor. However, phosphorylation at Thr75 will inhibit Thr34 phosphorylation. This selective, bidirectional regulation allows DARPP-32 to have a wide range of influence on downstream physiological effectors such as: ion pumps, neurotransmitter receptors, voltage-gate ion channels, and transcriptional factors. Studies suggest that it may also have a role in the Circadian System photic pathway. Overexpression of DARPP-32 has been linked with antiapoptotic mechanisms of cancer cells and has been observed in breast, colon, esophagus, gastric, and prostate cancer tissues.

Spécificité

This antibody recognizes DARPP-32 near the N-terminus.

Immunogène

KLH-conjugated linear peptide corresponding to mouse DARPP-32 near the N-terminus.

Application

Anti-DARPP-32 Antibody is an antibody against DARPP-32 for use in WB & IP.
Research Sub Category
Neurotransmitters & Receptors

Ion Channels & Transporters
Western Blot (Snap i.d.) Analysis: 1 µg/mL of this antibody from a previous lot detected DARPP-32 on 10 µg of rat brain tissue lysate.

Immunoprecipitaion Analysis: 10 µg of this antibody from a previous lot immunoprecipitated DARPP-32 from rat brain RIPA lysate.

Qualité

Evaluated by Western Blot on rat brain tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected DARPP-32 on 10 µg of rat brain tissue lysate.

Description de la cible

~ 32 kDa observed

Liaison

Replaces: AB1656

Autres remarques

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Despina E Ganella et al.
Frontiers in behavioral neuroscience, 11, 76-76 (2017-05-26)
Anxiety disorders are the most common type of mental disorder during adolescence, which is at least partly due to the resistance to extinction exhibited at this age. The dopaminergic system is known to be dysregulated during adolescence; therefore, we aimed
Inês T Afonso et al.
International journal of molecular sciences, 23(19) (2022-10-15)
Spinocerebellar ataxia type 2 (SCA2) is a rare autosomal, dominantly inherited disease, in which the affected individuals have a disease onset around their third life decade. The molecular mechanisms underlying SCA2 are not yet completely understood, for which we hypothesize
Stefanie L O'Neal et al.
Neurotoxicology, 44, 303-313 (2014-08-15)
Manganese (Mn) is an essential trace element, but excess exposure leads to accumulation in biological tissues, including the brain. Chronically high Mn levels in the brain are neurotoxic and can result in a progressive, irreversible neurological disorder known as manganism.
Silvana Obici et al.
Molecular metabolism, 4(10), 692-705 (2015-10-27)
Melanocortin-4 receptors (MC4Rs) are highly expressed by dopamine-secreting neurons of the mesolimbic tract, but their functional role has not been fully resolved. Voluntary wheel running (VWR) induces adaptations in the mesolimbic dopamine system and has a myriad of long-term beneficial
Maternal high-fat diet decreases milk endocannabinoids with sex-specific changes in the cannabinoid and dopamine signaling and food preference in rat offspring.
Dias-Rocha, et al.
Frontiers in Endocrinology, 14, 1087999-1087999 (2023)
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