233526

Coagulation Factor Xa, Human Plasma

Coagulation Factor Xa, Human Plasma, CAS 9002-05-5, is a native coagulation factor Xa prepared from Factor X by activation with Russel′s viper venom. The venom is removed after activation.

• Synonyme(s) : Coagulation Factor Xa, Human Plasma
• Numéro CAS: 9002-05-5
• Numéro de classification (Commission des enzymes): 3.4.21.6 (BRENDA, IUBMB)
• Numéro MDL: MFCD00163610
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Forme: lyophilized
• Activité spécifique: ≥170 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Solubilité: water: soluble
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Native coagulation factor Xa from human plasma prepared from homogeneous Factor X by activation with Russel’s viper venom. Hydrolyzes peptide and ester bonds at the carboxyl group of arginine. Specifically catalyzes the activation of prothrombin to thrombin. Prothrombin activation is accelerated by Factor Va, phospholipids, and Ca²⁺. Factor Xa was prepared from homogeneous Factor X, using Russels′ viper venom (RVV), which was removed after activation. Inhibited by AEBSF (Cat. No. 101500), DFP (Cat. No. 30967), PMSF (Cat. No. 52332), and soybean trypsin inhibitor (Cat. No. 650357). Sold on the basis of plasma equivalent units of Factor X. 100 plasma equivalent units are equal to 800 µg of Factor X.

Native coagulation factor Xa from human plasma prepared from homogeneous Factor X by activation with Russel’s viper venom. The venom is removed after activation. During coagulation, in the presence of Ca²⁺ and membrane phospholipids, a complex of Factor Xa and Factor Va (the prothrombinase complex) activates prothrombin to thrombin. Inhibitors include AEBSF (Cat. No. 101500), DFP (Cat. No. 30967), PMSF (Cat. No. 52332), and Soybean Trypsin Inhibitor (Cat. No. 650357). Sold on the basis of plasma equivalent units of Factor X. 100 plasma equivalent units are equal to 800 µg of Factor X.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol CHROMOZYM^® X substrate per min at 25°C.

Forme physique

Lyophilized from 700 mM NaCl, 20 mM Tris-HCl, pH 7.4.

Remarque sur l'analyse

homogeneous by SDS-PAGE

Autres remarques

Heeb, M.J., et al. 1994. Proc. Natl. Acad. Sci. USA91, 2728.
Lindhout, T., et al. 1994. Biochem. J.297, 131.
Morishita, H., et al. 1994. Thrombosis Res.73, 193.
Lupu, C., et al. 1993. Thrombosis Haemostasis70, 579.
Jordan, S.P., et al. 1992. Biochemistry31, 5374.
Schoen, P., et al. 1992. Br. J. Haemotol.81, 255.
Tracy, P.B., et al. 1992. Methods Enzymol.215, 329.
Lawson, J.H., and Mann, K.G. 1991. J. Biol. Chem.226, 11317.
Tans, G., et al. 1991. J. Biol. Chem.266, 21864.
Owen, W.G., et al. 1974. J. Biol. Chem.249, 594.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromozym is a registered trademark of Pentapharm AG, Basel

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable