14-864
PAK-1 PBD Protein (agarose free), 300 µg
For use in Affinity Binding Assays, ELISA & G-Protein Assays.
Synonyme(s) :
p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1
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About This Item
Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
human
Niveau de qualité
Poids mol.
Mw 21 kDa
Fabricant/nom de marque
Upstate®
Concentration
>50% (Coomassie blue staining, SDS-PAGE)
2 mg/mL
Technique(s)
ELISA: suitable
affinity binding assay: suitable (G-protein)
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Description générale
Product Source: GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1, expressed in E. coli.
Qualité
Routinely evaluated by G-protein activity assay
Forme physique
300 µg of PAK-1 PBD, GST in 150 µL of PBS, pH 7.4, 0.5 mM PMSF, containing 20% glycerol.
Stockage et stabilité
Stable for 1 year at -20C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Remarque sur l'analyse
Specific Activity: Affinity Precipitation Assay: 10 µg of this lot when added with 10 µL glutathione agarose, precipitated Rac-GTP from 3T3 and A431 cell lysates that were loaded in vitro with GTPγS. GDP treated of 3T3 and A431 lysates served as negative controls. Precipitated Rac-GTP was detected by immunoblot analysis using 1 &micor;g/mL anti-Rac (Catalog # 05-389) and secondary goat anti-mouse HRP antibody (Catalog # 12-349) on a Western Blot.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
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A Ros-Baro et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(21), 12050-12055 (2001-10-11)
It has been recently reported that insulin recruits a novel signaling machinery to lipid rafts required for insulin-stimulated GLUT4 translocation [Baumann, A., Ribon, V., Kanzaki, M., Thurmond, D. C., Mora, S., Shigematsu, S., Bickel, P. E., Pessin, J. E. &
A X Bigard et al.
European journal of applied physiology and occupational physiology, 76(5), 421-427 (1997-01-01)
We examined the extent of morphological alterations and the myosin heavy chain (MHC) distribution in the rat soleus muscle after a 4-week period of spontaneous recovery or retraining after hindlimb suspension (HS). Moreover, we tested the hypothesis that dantrolene sodium
Shamsideen A Ojelade et al.
PloS one, 10(9), e0137465-e0137465 (2015-09-15)
Responses to the effects of ethanol are highly conserved across organisms, with reduced responses to the sedating effects of ethanol being predictive of increased risk for human alcohol dependence. Previously, we described that regulators of actin dynamics, such as the
V Benard et al.
The Journal of biological chemistry, 274(19), 13198-13204 (1999-05-01)
A major function of Rac2 in neutrophils is the regulation of oxidant production important in bacterial killing. Rac and the related GTPase Cdc42 also regulate the dynamics of the actin cytoskeleton, necessary for leukocyte chemotaxis and phagocytosis of microorganisms. Although
Tudor I Oprea et al.
PloS one, 10(11), e0142182-e0142182 (2015-11-13)
Rho family GTPases (including Rac, Rho and Cdc42) collectively control cell proliferation, adhesion and migration and are of interest as functional therapeutic targets in numerous epithelial cancers. Based on high throughput screening of the Prestwick Chemical Library® and cheminformatics we
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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