UHPLC Gradient Analysis of Boswellia Herbal Supplement on Titan C18

extraction

1. Weigh 100 mg Bosewellia Herbal Supplement     2. Add 10 mL ethanol     3. Sonicate 30 min at 40°C     4. Dilute 100 μL extract, 400 μL ethanol, 500 μL water

column

Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)

mobile phase

[A] 0.1% phosphoric acid; [B]acetonitrile

gradient

40% B for 0.5 min; to 100% B in 2 min; held at 100% B for 10 min

flow rate

0.4 mL/min

pressure

4050 psi (279 bar)

column temp.

35 °C

detector

UV, 210 nm

injection

2 μL

sample

Bosewellia Extract in 50:50, water:ethanol