819697

TSKgel^® Amide-80 (5 µm) HPLC Columns

L x I.D. 25 cm × 2 mm, carbamoyl phase

• Synonyme(s) : TSKgel^® Amide-80, 25 cm x 2 mm ID, 5 μm
• Code UNSPSC : 41115711
• eCl@ss : 32110501

Propriétés

• product name: TSKgel^® Amide-80 HPLC Column, phase carbamoyl, L × I.D. 25 cm × 2 mm, 5 μm particle size
• Matériaux: stainless steel hardware
• Agence: suitable for USP L68
• Gamme de produits: TSKgel^®
• Fabricant/nom de marque: Tosoh 19697
• Paramètres: 0-100% organic solvent; 10-80 °C temperature; 150 bar max. pressure
• Technique(s): HPLC: suitable
• L × D.I.: 25 cm × 2 mm
• Superficie: 450 m²/g
• Matrice: silica particle platform
• Groupe de la matrice active: carbamoyl phase
• Taille des particules: 5 μm
• Dimension de pores: 100 Å
• pH de fonctionnement: 2.0-7.5
• Technique de séparation: hydrophilic interaction (HILIC)

Description

Description générale

TSKgel Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications.

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Informations légales

TSKgel is a registered trademark of Tosoh Corporation