T7924

Transforming Growth Factor-α human

TGF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonyme(s) : hTGF-α, TGF-α
• Formule empirique (notation de Hill): C₂₃₉H₃₄₈N₇₀O₇₂S₆
• Numéro CAS: 105186-99-0
• Poids moléculaire : 5546.14
• Numéro MDL: MFCD00082077
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (SDS-PAGE and HPLC)
• Forme: powder
• Puissance: ≤0.2 ng/mL ED₅₀
• Qualité: endotoxin tested
• Poids mol.: 5.5 kDa
• Conditionnement: pkg of 1 mg; pkg of 5 mg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1 EU/μg
• Numéro d'accès UniProt: P01135
• Température de stockage: −20°C
• Informations sur le gène: human ... TGFA(7039)

Description

Amino Acid Sequence

Val-Val-Ser-His-Phe-Asn-Asp-Cys-Pro-Asp-Ser-His-Thr-Gln-Phe-Cys-Phe-His-Gly-Thr-Cys-Arg-Phe-Leu-Val-Gln-Glu-Asp-Lys-Pro-Ala-Cys-Val-Cys-His-Ser-Gly-Tyr-Val-Gly-Ala-Arg-Cys-Glu-His-Ala-Asp-Leu-Leu-Ala [Disulfide bridges: 8-21; 16-32; 34-43]

Description générale

The TNFα (tumor necrosis factor α) gene is mapped to human chromosome 6p21.33. TNFα is a member of TNF superfamily.

Application

Transforming Growth Factor-α human has been used:

• to determine the ideal concentration of growth factors to be used in the culture medium that supports sheep preantral follicles
• in human hepatocytes cell culture and keratinocyte cell culture
• to induce morphogenesis in primary mouse organoids

Actions biochimiques/physiologiques

The TNFA gene is significantly associated with the regulation of innate and adaptive immune responses. Polymorphism of TNFA gene is known to cause acute motor axonal neuropathy. The cytokine release and gene action is associated with epithelial cells, myeloid cells, endothelial, as well as tumor cells. Tumor cells are known to release TNFA, specifically in response to chemotherapy.

Transforming growth factor-α (TGF-α), is a 5.5 kDa polypeptide containing 50 amino acids in its mature form. It was originally identified as an agent that reversibly confers a transformed phenotype upon normal non-neoplastic cells, such as normal rat kidney fibroblasts. This activity requires the presence of transforming growth factor-β (TGF-β), which potentiates the action of TGF-α via a separate receptor. TGF-α is synthesized by monocytes, keratinocytes and many tissues and tumors. Mice genetically engineered to be lacking TGF-α expression showed no abnormalities except for the detection of a "wavy" hair coat. TGF-α exerts its action through the EGF receptor.

Forme physique

Lyophilized from a 0.2 μm filtered buffered solution.

Remarque sur l'analyse

The biological activity is measured by its ability to stimulate ³H-thymidine incorpation into BALB/c 3T3 cells.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)