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T3934

Sigma-Aldrich

Réactif TRI®

LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid.

Synonyme(s) :

Réactif TRI® - Réactif d'isolement de l'ARN

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About This Item

Numéro MDL:
MFCD00213058
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.52

Utilisation

0.75 mL sufficient for 0.25 mL fluid samples

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Description générale

TRI Reagent LS is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA, and protein from liquid samples of human, animal, plant, yeast, bacterial, and viral origin.

A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of liquid samples. TRI Reagent LS performs well with large or small sample volumes, and many samples can be simultaneously extracted.

TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase solution. When a biological sample is homogenized or lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI Reagent LS processes 0.25 ml of a liquid sample such as amniotic fluid.

This is one of the most effective methods for isolating total RNA from fresh samples in only one hour. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.

Application

TRI Reagent® has been used in RNA extraction.
The reagent has been used for isolating total RNA. The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

Caractéristiques et avantages

  • Easily scalable RNA isolation
  • Works with many sources: human, plant, yeast, bacterial, or viral
  • Better yields than traditional guanidine thiocyanate/cesium chloride methods

Informations légales

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Organes cibles

Nervous system,Kidney,Liver,Skin

Risques supp

EUH032

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

174.2 °F - closed cup

Point d'éclair (°C)

79 °C - closed cup

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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TGF beta Presence During IgE-dependent Sensitization Primes Mast Cells for Higher VEGF Production After Fc varepsilon RI Activation
Benitez-GJP, et al.
Open Allergy Journal, 2, 16-26 (2009)
Cyclic dinucleotides modulate induced type I IFN responses in innate immune cells by degradation of STING
Rueckert C, et al.
Faseb Journal (2017)
TGF Presence During IgE-dependent Sensitization Primes Mast Cells for Higher VEGF Production After Fc RI Activation
Garrido
Open Allergy Journal (2009)
Nicola Jeffery et al.
Cell & bioscience, 11(1), 144-144 (2021-07-25)
Beta cell identity changes occur in the islets of donors with diabetes, but the molecular basis of this remains unclear. Protecting residual functional beta cells from cell identity changes may be beneficial for patients with diabetes. A somatostatin-positive cell population
Raul Y Sanchez David et al.
eLife, 5, e11275-e11275 (2016-03-25)
The RIG-I-like receptors (RLRs) play a major role in sensing RNA virus infection to initiate and modulate antiviral immunity. They interact with particular viral RNAs, most of them being still unknown. To decipher the viral RNA signature on RLRs during
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