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RAB0508

Sigma-Aldrich

Human VEGF ELISA Kit

for cell and tissue lysates

Synonyme(s) :

VEGF-A, Vascular endothelial growth factor A 

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1 KIT
795,00 $

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1 KIT
795,00 $

About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

795,00 $


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Devis pour commande en gros

Espèces réactives

human

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable
capture ELISA: suitable

Entrée

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... VEGFA(7422)

Comparer avec des articles similaires

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1 of 4

Cet article
RAB0513RAB0195RAB0190
Gene Information

human ... VEGFA(7422)

Gene Information

human ... VEGFC(7424)

Gene Information

human ... FLT4(2324)

Gene Information

human ... FIGF(2277)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 13.72-10000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 12.29-3000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.2 ng/mL
standard curve range: 0.313-20 ng/mL

input

sample type cell lysate
sample type tissue lysate

input

sample type cell culture supernatant(s)
sample type serum
sample type urine
sample type plasma

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma
sample type urine

input

sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

Description générale

The Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF in cell lysate and tissue lysate.

Immunogène

Recombinant Human VEGF-A

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Actions biochimiques/physiologiques

VEGF (vascular endothelial growth factor) is a potent angiogenic cytokine. It stimulates proliferation and migration of endothelial cells. VEGF induces microvascular permeability by enhancing function of vesicular-vacuolar organelle (VVO). VEGF plays a prominent role in both normal and pathological angiogenesis.[1] Substantial evidence suggests that VEGF is involved in the induction of tumor metastasis and intraocular neovascular syndromes.[2][3] Increased expression of VEGF causes retinal angiogenesis, thus, inhibition of VEGF using soluble VEGF-receptor chimeric proteins can be considered as a potential therapy for ischemic retinal disease.[3] Polymorphism in VEGF at C (-634) G is linked with diabetic retinopathy in type 2 diabetes. Altered expression of VEGF is also associated with rheumatoid arthritis, Graves′ disease, psoriasis, cardiovascular disease, and peripheral limb disease.[2]

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS

  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  • RABLYSIS12X Cell Lysis Buffer (Item J)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictogrammes

Corrosion

Mention d'avertissement

Warning

Mentions de danger

Conseils de prudence

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials


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Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Miyako Kurihara-Shimomura et al.
International journal of oncology, 56(5), 1093-1100 (2020-04-23)
Mast cells (MCs) are present in the tumor stroma, and MCs that express the mast cell‑specific proteases tryptase and chymase (MCTC) exhibit several tumor‑related functions. It was previously reported that melanoma inhibitory activity (MIA) gene family members, including MIA, MIA2
Suppression of retinal neovascularization in vivo by inhibition of vascular endothelial growth factor (VEGF) using soluble VEGF-receptor chimeric proteins.
Aiello LP
Proceedings of the National Academy of Sciences of the USA, 92, 10457-10461 (1995)
Vascular permeability factor/vascular endothelial growth factor: a multifunctional angiogenic cytokine.
Brown LF
EXS, 79, 233-269 (1997)
A common polymorphism in the 5'-untranslated region of the VEGF gene is associated with diabetic retinopathy in type 2 diabetes.
Awata T
Diabetes, 51, 1635-1639 (2002)
Piyush Gondaliya et al.
PloS one, 13(11), e0208044-e0208044 (2018-11-30)
The role of DNA methylation has not been enough explored in pathophysiology of diabetic nephropathy (DN). However, according to recent reports it has been inferred that hypermethylation could be one of the principle cause associated with the enhancement of DN.

Questions

  1. can this kit be used for cell culture supernatants as well? if the same sensitivity is required and if the lysis buffer is not used...

    1 answer
    1. This kit has been optimized with cell lysate and tissue lysate as the input sample types. If you are interested in a kit that can be used for cell culture supernatants, we recommend RAB0507 which is designed for serum, plasma, cell culture supernatants and urine samples.

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