R9894
Ras human
Wild type, recombinant, expressed in E. coli, buffered aqueous glycerol solution
Synonyme(s) :
H-Ras, rH-RAS, WT
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About This Item
Produits recommandés
Produit recombinant
expressed in E. coli
Pureté
≥80% (SDS-PAGE)
Forme
buffered aqueous glycerol solution
Poids mol.
28 kDa
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−70°C
Informations sur le gène
human ... RASGEF1B(153020)
Description générale
Ras proto-oncogene is a guanine-nucleotide binding protein that couples tyrosine kinase receptor signals to mitogen activated protein (MAP) kinase cascade. It has an important role in cell growth. The Ras gene is localized on human chromosome 11p15.5. Ras is a substrate for farnesyltransferase (FTase) and when farnesylated, it is anchored to the membrane.
Application
Human Ras has been used as a negative control in tubulin overlay assay.
Actions biochimiques/physiologiques
Ras proteins, p21, are guanyl nucleotide binding proteins that play an important role in cellular signal transduction. Ras proteins are regulatory proteins that bind GTP in their active state. A Ras intrinsic GTPase activity hydrolyzes guanosine-5′-triphosphate (GTP) to guanosine diphosphate (GDP) thus inactivating Ras, while activation of Ras requires exchange of the bound GDP with GTP. Inhibition of Ras activity by stimulation of its GTPase activity is enhanced by GTPase activating proteins. Membrane localization of Ras is crucial for its cellular activity. Inhibition of Ras farnesylation is a target for anti-cancer drug development, since abnormally active Ras is present in more than 50% of human cancers.
Conditionnement
Package size based on protein content
Forme physique
Solution in 50% glycerol containing 50 mM Tris (pH 8.0), 5 mM MgCl2, 45 mM NaCl, 1 mM DTT
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Real-time in vitro measurement of intrinsic and Ras GAP-mediated GTP hydrolysi
Shutes A, et al.
Test (2006)
Human Mucin Gene MUC5B, the 10.7-kb Large Central Exon Encodes Various Alternate Subdomains Resulting in a Super-repeat
Jean-Luc D
The Journal of Biological Chemistry (1997)
Inactivation of Ras GTPase-activating proteins promotes unrestrained activity of wild-type Ras in human liver cancer
Calvisi DF, et al.
Journal of Hepatology, 54(2), 311-311 (2011)
A role for the vesicle-associated tubulin binding protein ARL6 (BBS3) in flagellum extension in Trypanosoma brucei
Helen P.Price
Biochim. Biophys. Acta Gen. Subj. (2012)
Ras protein farnesyltransferase: A strategic target for anticancer therapeutic development.
Journal of Clinical Oncology (1999)
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