R4503
Sma I from Serratia marcescens Sb
Restriction Enzyme
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
Produits recommandés
Qualité
for molecular biology
Forme
buffered aqueous glycerol solution
Concentration
10,000 units/mL
Conditions d'expédition
wet ice
Température de stockage
−20°C
Spécificité
Recognition sequence: 5′-CCC/GGG-3′
Cutting results: a 2-10-fold Sma I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes.
Cutting results: a 2-10-fold Sma I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes.
Application
SmaI is a restriction endonuclease used to cut DNA at the recognition sequence 5′-CCC/GGG-3′, generating DNA fragments with blunt termini.
Autres remarques
Supplied with 10x Restriction Enzyme Buffer SA (B7531).
Comment: Half-life of Sma I at 37 °C is approximately 15 minutes.
Liaison
Isoschizomer: Xma I. Unlike Xma I, Sma I produces blunt-ended fragments.
Définition de l'unité
One unit is the enzyme activity that completely cleaves 1 mg of λDNA in 1 hr. at 25 °C in a total volume of 25 ml of 1x digestion buffer SA forrestriction enzymes.
Forme physique
Solution in 10 mM potassium phosphate, pH 7.0, 1 mM EDTA, 300 mM NaCl, 5 mM dithiothreitol, 50% glycerol (v/v) , 0.02% polydocanol (v/v), at 4 °C
Produit(s) apparenté(s)
Tampon d'incubation
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
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Leila C Campos et al.
Diagnostic microbiology and infectious disease, 65(2), 112-115 (2009-08-28)
The newly described Streptococcus pneumoniae serotype 6C accounted for 2.3% (16/709) of meningitis cases and 3.2% (3/95) of nasopharyngeal isolates from healthy individuals in Brazil. The strains were multidrug resistant (18.8%) and genetically diverse. Despite low serotype 6C prevalence, continuous
D Savoia et al.
The Journal of antimicrobial chemotherapy, 45(1), 41-47 (2000-01-11)
Two hundred and twenty one Streptococcus pyogenes isolates collected from throat swabs of untreated children with uncomplicated pharyngotonsillitis living in two centres situated in the north of Italy were tested to evaluate their macrolide resistance phenotype. Isolates were also typed
Two restriction-like enzymes from Xanthomonas malvacearum.
S A Endow et al.
Journal of molecular biology, 112(3), 521-529 (1977-05-25)
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
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