MAK072
Pyruvate Kinase Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
sufficient for 100 colorimetric or fluorometric tests
Méthode de détection
colorimetric
fluorometric
Maladie(s) pertinente(s)
hematological disorder; cancer
Température de stockage
−20°C
Description générale
Pyruvate kinase (PK) is an enzyme that catalyzes the final step of glycolysis, the transfer of a phosphate group from phospho(enol)pyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase deficiency, due to defects in pyruvate kinase expression or activity, is the second most common cause of hemolytic anemia.
The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.
The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.
Application
Pyruvate Kinase Activity Assay Kit has been used to determine the activity of pyruvate kinase in samples.
Adéquation
Suitable for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc
Principe
The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.
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Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1
Code de la classe de stockage
10 - Combustible liquids
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