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SRP0415

Sigma-Aldrich

PKM1 active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonyme(s) :

OIP3, PK3, PKM2, TCB, pyruvate kinase, muscle

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About This Item

Numéro CAS:
1644286-11-2
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥80% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

59 kDa

Conditionnement

pkg of 20 μg

Fabricant/nom de marque

Sigma-Aldrich

Conditions de stockage

avoid repeated freeze/thaw cycles

Concentration

1.8 mg/mL

Technique(s)

cell based assay: suitable

Solubilité

water: soluble

Numéro d'accès NCBI

NM_182470

Numéro d'accès UniProt

P14618

Application(s)

life science and biopharma

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... PKM(5315)

Description générale

Research area: Cell Signaling

Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23.

Application

PKM1active human has been used in:
  • cell-based kinase assay to study the ability of a compound to inhibit the kinase activity of PKM1.
  • oxidative stress experiments on PKM2.
  • Co-immunoprecipitation (Co-IP) to determine pyruvate kinase M2 (PKM2) as a novel direct binding partner of phosphoserine aminotransferase 1 (PSAT1).

Actions biochimiques/physiologiques

Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H360D

Classification des risques

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

C Parkison et al.
Biochemical and biophysical research communications, 179(1), 668-674 (1991-08-30)
Using a T7 expression system, the monomer of rat pituitary pyruvate kinase, subtype M1 (PKM1), was overexpressed in Escherichia coli and purified to homogeneity. The monomeric p58-M1 has intrinsic enzymatic activity with a Vmax of 79 +/- 20 units/mg and
Nuclear Pyruvate Kinase M2 (PKM2) Contributes to Phosphoserine Aminotransferase 1 (PSAT1)-Mediated Cell Migration in EGFR-Activated Lung Cancer Cells
Biyik-Sit R, et al.
Cancer, 13(16), 3938-3938 (2021)
Evaluation of serum and pleural levels tumor M2-pyruvate kinase in lung cancer patients with pleural effusion
Tiantian Z, et al.
BMC Pulmonary Medicine, 22, 307-307 (2022)
Development of Novel Irreversible Pyruvate Kinase M2 Inhibitors
I-Shan H, et al.
Journal of Medicinal Chemistry, 62(18), 8497-8510 (2019)
Research progress on the role of PKM2 in the immune response
Chunyan L, et al.
Frontiers in Immunology (2022)
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