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L7381

Sigma-Aldrich

Lectin from Arachis hypogaea (peanut)

FITC conjugate, lyophilized powder

Synonyme(s) :

PNA, Peanut agglutinin

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Conjugué

FITC conjugate

Niveau de qualité

200

Forme

lyophilized powder

Composition

Protein, ~10% Lowry

Ampleur du marquage

4-8 mol FITC per mol protein

Température de stockage

−20°C

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Catégories apparentées

Description générale

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Application

Lectin from Arachis hypogaea (peanut) has been used:
  • to determine the acrosomal status (presence or absence of acrosomal matrix corresponding to intact or acrosome-reacted spermatozoa) on viable sperm cells
  • to probe cryosections of mediastinal lymph nodes (mLNs) with fluorochrome-labeled PNA and anti-B220 to detect germinal centers (GCs)
  • to visualize the cone outer segments of pre-treated retinal sections

Actions biochimiques/physiologiques

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.
PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.

Forme physique

Contains phosphate buffer salts and NaCl

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Centimorgan-range one-step mapping of fertility traits using interspecific recombinant congenic mice
Lhote D, et al.
Genetics, 176(3), 1907-1921 (2007)
Interleukin-2 inhibits germinal center formation by limiting T follicular helper cell differentiation
Ballesteros Tato A, et al.
Immunity, 36(5), 847-856 (2012)
NeuroD1 regulates expression of thyroid hormone receptor beta2 and cone opsins in the developing mouse retina
Liu H, et al.
The Journal of Neuroscience, 28(3), 749-756 (2008)
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Cryobiology, 74, 8-12 (2017-01-04)
Little is known about the effects of Cholesterol-Loaded Cyclodextrin (CLC) on post-thaw semen quality in chicken. The aim of the present study is to investigate the efficacy of CLC levels (0, 1, 2 and 3 mg/mL Schramm diluent) on post-thawed semen
Caner Öztürk et al.
Acta veterinaria Hungarica, 65(3), 429-439 (2017-09-29)
The present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and
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