H8509
β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides
Type V, lyophilized powder, 250-750 units/mg protein
Synonyme(s) :
(R)-3-Hydroxybutanoate:NAD+ oxidoreductase, 3-HBDH
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25 UNITS
1 580,00 $
1 580,00 $
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25 UNITS
1 580,00 $
About This Item
1 580,00 $
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros
Produits recommandés
Type
Type V
Forme
lyophilized powder
Activité spécifique
250-750 units/mg protein
Produit purifié par
chromatography
Activité étrangère
lactate dehydrogenase ≤0.05%
malate dehydrogenase ≤0.1%
Température de stockage
−20°C
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Application
Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).
Actions biochimiques/physiologiques
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
Définition de l'unité
One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.
Forme physique
Lyophilized powder containing Tris buffer salts
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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