We do not recommend re-using the columns. Each column is intended for one time use.
H7787
HIS-Select® Spin Columns
Synonyme(s) :
His-Tag Spin Columns
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10 EA
251,00 $
50 EA
894,00 $
251,00 $
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Changer de vue
10 EA
251,00 $
50 EA
894,00 $
About This Item
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56
251,00 $
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Produits recommandés
Durée de conservation
2 yr at 2‑8 °C
Niveau de qualité
Dimension de pores
100 nm pore size
Capacité
>0.5 mg/column
Température de stockage
2-8°C
Description générale
Kit contains 3 collection tubes for every spin column.
Application
Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.
Caractéristiques et avantages
- High Selectivity for higher purity
- Convenient and Ready-to-Use
- Consistent protein binding each use
- Compatible with CelLytic™ cell lysis reagents
Informations légales
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
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Streptomyces are filamentous bacteria with a complex developmental life cycle characterized by the formation of spore-forming aerial hyphae. Transcription of the chaplin and rodlin genes, which are essential for aerial hyphae production, is directed by the extracytoplasmic function (ECF) σ
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In Escherichia coli, the ammonia channel AmtB and the P(II) signal transduction protein GlnK constitute an ammonium sensory system that effectively couples the intracellular nitrogen regulation system to external changes in ammonium availability. Binding of GlnK to AmtB apparently inactivates
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Can HIS-Select® Spin Column, Product H7787, be re-used?
1 answer-
Helpful?
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What is the binding capacity of the HIS-Select® Spin Column, Product H7787?
1 answer-
The binding capacity of a HIS-Select Spin Column is >500 ug per column as determined witha ~30 kDa histidine tagged protein recovered under high levels of protein expression.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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