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GERPN1520B

Hybond®-N+ hybridization membranes

W × L 15 cm × 20 cm, sheets, pack of 10 ea

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About This Item

Code UNSPSC :
41105339
Nomenclature NACRES :
NB.22

Matériaux

positively charged nylon
sheets

Forme

solid

Conditionnement

pack of 10 ea

Fabricant/nom de marque

Cytiva RPN1520B

Larg. × L

15 cm × 20 cm

Couleur

White

Dimension de pores

0.45 μm pore size

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Description générale

Hybond™N+ is a high sensitivity positively charged nylon membrane for DNA and RNA blotting with nucleic acid binding capacity and high tensile strength.

Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation gives better reproducibility. Hybond-N+ is not suitable for alkali blotting and fixation. All Hybond membranes are manufactured specifically for life science applications. Production runs are carefully controlled and the product exhaustively screened to ensure that only the most consistent product reaches the user. All Hybond membranes are identical on both sides. Supported nylon membranes have a high binding capacity for nucleic acid, in addition to high tensile strength. For applications requiring a high degree of sensitivity and/or reprobing these types of membranes are an ideal choice. Due to its high protein binding capacity, nylon requires extensive blocking prior to detection with antibodies to avoid high backgrounds. Consequently, nylon membranes are not recommended for use in Western blotting.

Application

Hybond™-N+ hybridization membranes are ideal to use with:

  • Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm² recommended for use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems.
  • All membrane arrays with a high density.
  • Applications that need high sensitivity and/or probing.

Caractéristiques et avantages

  • On both sides, all Hybond™membranes are identicalIncompatible with alkali blotting and fixation.
  • To ensure that only the most consistent product reaches the user, production runs are carefully monitored, and the product is thoroughly screened.
  • Specially designed for life science applications.
  • In conventional southern blotting, produces excellent sensitivity.
  • To prevent high background sides, nylon needs to be extensively blocked before being detected with antibodies due to its high protein binding capacity.
  • Not suitable for western blotting.

Informations légales

Hybond is a registered trademark of Cytiva

Code de la classe de stockage

13 - Non Combustible Solids


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