FLAGIPT1
FLAG® Immunoprecipitation Kit
Synonyme(s) :
Anti-ddddk, Anti-dykddddk
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32
Produits recommandés
Utilisation
kit sufficient for 50 reactions
Niveau de qualité
Classe(s) chimique(s) de l'analyte
proteins (FLAG® Affinity Gels)
Technique(s)
immunoprecipitation (IP): suitable
Conditions d'expédition
wet ice
Température de stockage
−20°C
Description générale
Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.
Application
Learn more product details in our FLAG® application portal.
Caractéristiques et avantages
- Utilizes highly specific ANTI-FLAG M2 affinity gel
- No preliminary steps or calibrations
- 3xFLAG™ peptide provides efficient and gentle elution through direct competition
Autres remarques
All prodedures to be performed at 2-8°C.
Informations légales
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3 - Eye Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
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