F1879
Formaldehyde Dehydrogenase from Pseudomonas sp.
lyophilized powder, 1.0-6.0 units/mg solid
Synonyme(s) :
FDH, Formaldehyde:NAD+ oxidoreductase
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Produits recommandés
Forme
lyophilized powder
Activité spécifique
1.0-6.0 units/mg solid
Température de stockage
−20°C
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Description générale
Formaldehyde Dehydrogenase (FDH) belongs to the medium-chain alcohol dehydrogenase family. It is encoded by the fdhA and fdhB gene in Pseudomonas aeruginosa. The FDH sequences of P. aeruginosa and P. putida are conserved and show homology. FDH exists as dimer and tetramer with zinc in their catalytic active site. The monomer corresponds to 350-400 amino-acid residues.
Application
Formaldehyde Dehydrogenase from Pseudomonas sp. has been used:
- For standard curve generation for the demethylation-FDH assay of lysine specific demethylase 1 (LSD1) and in formaldehyde dehydrogenase (FDH) assay.
- As a biosensor for the presence of formaldehyde in pharmaceuticals, waste water, vaccines and industrial products.
- In coupled pectin methyl esterase (PME) enzyme assay.
Actions biochimiques/physiologiques
Formaldehyde dehydrogenase (FDH) is an enzyme that catalyzes the oxidation of formaldehyde to formate with NAD+ as an electron acceptor.Formaldehyde dehydrogenases are involved in the biological detoxification of formaldehyde, a toxic product in the metabolism of compounds like histidine, vanillate etc. They detoxify by converting formaldehyde to carbon-di-oxide. FDH plays a crucial role in regulating protein S-nitrosation in mammalian cells by decomposing S-nitrosoglutathione. They are also involved in the regulation of nitrosothiol homeostasis by catalyzing the reduction of endogenous nitrosylating agent S-nitrosoglutathione (GSNO).
Définition de l'unité
One unit will oxidize 1.0 μmole of formaldehyde to formic acid per min at pH 7.5 at 37 °C.
Forme physique
Lyophilized powder containing ~70% stabilizers as Mg2+, Ca2+, bovine serum albumin, glycine, and lysine
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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Les clients ont également consulté
The role of a formaldehyde dehydrogenase-glutathione pathway in protein S-nitrosation in mammalian cells
Haqqani A S, et al.
Nitric Oxide, 9(3), 172-181 (2003)
Structure of formaldehyde dehydrogenase from Pseudomonas aeruginosa: the binary complex with the cofactor NAD+
Liao Y, et al.
Acta Crystallographica. Section F, Structural Biology Communications, 69(9), 967-972 (2013)
Expression, purification, and characterization of formaldehyde dehydrogenase from Pseudomonas aeruginosa
Zhang W, et al.
Protein Expression and Purification, 92(2), 208-213 (2013)
Ivan V Rosado et al.
Nature structural & molecular biology, 18(12), 1432-1434 (2011-11-15)
Metabolism is predicted to generate formaldehyde, a toxic, simple, reactive aldehyde that can damage DNA. Here we report a synthetic lethal interaction in avian cells between ADH5, encoding the main formaldehyde-detoxifying enzyme, and the Fanconi anemia (FA) DNA-repair pathway. These
Redundancy of enzymes for formaldehyde detoxification in Pseudomonas putida
Roca A, et al.
Journal of Bacteriology, 191(10), 3367-3374 (2009)
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