Accéder au contenu
MilliporeSigma
Toutes les photos(1)

Principaux documents

E8029

Sigma-Aldrich

E-Toxate™ Endotoxin standard

for endotoxin quantitation

Synonyme(s) :

E. coli 0.55:B5 LPS standard

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme

Sélectionner une taille de conditionnement

1 VIAL
90,80 $

90,80 $

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Toutes les photos(1)

Sélectionner une taille de conditionnement

Changer de vue
1 VIAL
90,80 $

About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.24

90,80 $

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.

Contient

solubilizer

Température de stockage

2-8°C

Catégories apparentées

Application

E-Toxate endotoxin standard was used along with the limulus amebocyte lysate assay kit to determine the concentration of endotoxins in isolated samples of human holotransferrin and apotransferrin used to treat primary hepatocyte cultures. [1]

Autres remarques

A preparation of E. coli 055:B5 lipopolysaccharide
Can be stored at 2-8 °C when stored separately from the E-Toxate kit.

Remarque sur l'analyse

Standardized against USP reference standard endotoxin (RSE)

Informations légales

E-Toxate is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000429783
0000429783
0000397691
0000397691
0000331446

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Anna Scanu et al.
International journal of molecular sciences, 23(21) (2022-11-12)
We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation. In CIA, mice were intraperitoneally injected with PBS, HA, or BPI plus or minus
Akrivi Chrysanthopoulou et al.
Clinical immunology (Orlando, Fla.), 247, 109240-109240 (2023-01-25)
Kruppel-like factor 2 (KLF2) has been linked with fibrosis and neutrophil-associated thromboinflammation; however, its role in COVID-19 remains elusive. We investigated the effect of disease microenvironment on the fibrotic potential of human lung fibroblasts (LFs) and its association with KLF2
Lan Lin et al.
Blood, 110(6), 2182-2189 (2007-06-02)
The peptide hormone hepcidin is the principal regulator of systemic iron homeostasis. We examined the pathway by which iron stimulates the production of hepcidin. In humans who ingested 65 mg of iron, the increase in transferrin saturation preceded by hours
Akrivi Chrysanthopoulou et al.
JCI insight, 6(18) (2021-07-30)
Innate immunity and chronic inflammation are involved in atherosclerosis and atherothrombosis, leading to target organ damage in essential hypertension (EH). However, the role of neutrophils in EH is still elusive. We investigated the association between angiotensin II (Ang II) and

Questions

1–6 of 6 Questions  

  1. Hi, For E8029 endotoxin quantification product, 1. Is it a powder or liquid? 2. Solubility in water if it's a powder? How much water is needed to prepare a solution? Thanks,

    1 answer

    1. This product is a lyophilized powder typically used as a standard in the E-Toxate Endotoxin kits. See the link below to review these kits. For use with these kits, the material is reconstituted in endotoxin-free water at 4000UE/mL. Each vial contains a range of 10,000 - 20,000 units which is reported in the lot specific Certificate of Analysis. The mass weight is not determined. However, the amount of powder may be estimated based on the conversion factor of 5 EU per nanogram.
      Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/e8029#product-documentation

      Please see the link below to review the E-Toxate Endotoxin Kit products:
      https://www.sigmaaldrich.com/substance/etoxatekit1234598765

      Please see the link below to review the kit protocol with reconstitution instructions for this standard:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/378/800/et0200-pis-ms.pdf

      Helpful?

  2. What is the amount of the powder in each vial in grams or milligrams?

    1 answer

    1. The product comes as 10000 - 20000 EU Endotoxin per vial. Unfortunately, the exact weight information is not available.

      Helpful?

  3. How much water should I use to reconstitute my E-Toxate™ Endotoxin standard, Product E8029?

    1 answer

    1. Each batch of endotoxin standard varies slightly in its activity so the amount of water to use may vary from lot to lot.  The certificate of analysis will give the amount of endotoxin units in the vial, so that the end user may calculate the appropriate amount of water to add to the vial in order to reconsitute to a final concentration of 4000 EU/mL.  The certificate of analysis for each lot may be retrieved from our website.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What is the E-Toxate™ Endotoxin standard, Product E8029?

    1 answer

    1. The endotoxin standard is purified from a crude extract of lipopolysaccharide from E. coli 055:B5 and standardized against the current USP reference standard.  It is reconstituted with an appropriate amount of water to yield a 4000 EU/mL solution.

      Helpful?

  6. How is the E-Toxate™ Endotoxin standard, Product E8029, dissolved?

    1 answer

    1. E8029 is reconstituted in endotoxin-free water to a concentration of 4000 EU/mL.  Reconstituted standard solutions can be stored at 2 - 8°C for at least 2 weeks.

      Helpful?

Reviews

No rating value

Active Filters

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique