D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

• Synonyme(s) : DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
• Numéro CAS: 9003-98-9
• Numéro de classification (Commission des enzymes): 3.1.21.1 (BRENDA, IUBMB)
• Numéro CE : 232-667-0
• Numéro MDL: MFCD00130918
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.53

Propriétés

• Source biologique: bovine pancreas
• Qualité: for molecular biology
• Forme: buffered aqueous glycerol solution
• Activité spécifique: 10000 units/mg protein
• Poids mol.: 29.1 kDa
• Concentration: 5—15 mg protein/mL
• Technique(s): DNA extraction: suitable
• Adéquation: suitable for molecular biology
• Numéro d'accès UniProt: P00639
• Application(s): cell analysis
• Activité étrangère: RNase, none detected
• Température de stockage: −20°C
• Informations sur le gène: cow ... DNASE1(282217)

Description

Description générale

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg²⁺, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn²⁺, both strands can be cleaved.

Application

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Composants

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

Définition de l'unité

One unit will cause a ΔA₂₆₀ of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

Autres remarques

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)